EGCG Crosslinked Biomimetic Mineralized Decellularized Matrix of Filefish Skin As A Membrane for Guided Bone Tissue Regeneration
Objective:To construct decellularized matrix of filefish skin and crosslink it to be biomimetic minerali-zation template as a guided bone regeneration membrane.Methods:Filefish skin of marine origin was selected,and the decellularized matrix of filefish skin(filefish skin decellularized matrix,FS-ECM)was prepared by a combined physicochemical method.The structural features of FS-ECM were preliminarily explored by HE staining,scanning electron microscope(SEM),X-ray diffraction(XRD),and water contact angle.Then,the cross-linking modifica-tion of epigallocatechin gallate(EGCG)was utilized(EGCG crosslinked filefish skin decellularized matrix,E-FS-ECM)to improve the material's anti-enzymatic and mechanical properties.The EGCG-collagen in vitro biomimetic mineralization template(EGCG crosslinked bio-mimetic mineralized filefish skin decellularized matrix,EB-FS-ECM)was constructed through the modification of EGCG,and the collagen biomimetic mineralization strategy was used to further improve the various properties.The differences in the physicochemical properties of the materials before and after modification were evaluated using SEM,mapping,water contact angle,elastic modulus,thermo-gravimetric analysis,and in vitro degradation.Results:There was no significant difference between the surface structural properties of FS-ECM at different sites(P＞0.05),and FS-ECM contained a certain amount of hydroxyapatite crystals on the outer surface.After the modification by EGCG cross-linking and construction of bio-mimetic mineralization templates,the hydrophilicity,mechanical strength,thermal stability,and resistance to en-zymatic degradation of E-FS-ECM and EB-FS-ECM were significantly higher than those of FS-ECM(P＜0.05).Conclusion:EGCG cross-linking and biomimetic mineralization significantly improved the hydrophilicity,thermal stability,and mechanical strength,and slowed down the degradation rate of FS-ECM.Decellularized matrix of file-fish skin through modification is expected to be an suitable material for GBR membranes.
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