首页|Dnmt3b对髁突纤维软骨干细胞多向分化调控作用的实验研究

Dnmt3b对髁突纤维软骨干细胞多向分化调控作用的实验研究

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目的:探究DNA甲基转移酶3b(DNA methyltransferase 3b enzyme,Dnmt3b)对大鼠髁突纤维软骨干细胞(fibrocartilage stem cells,FCSCs)成脂、成骨、成软骨向分化能力的影响.方法:体外分离培养大鼠髁突FCSCs,构建Dnmt3b过表达慢病毒,感染FCSCs建立过表达Dnmt3b的FCSCs细胞模型,并对其进行成脂、成骨、成软骨诱导培养,检测Dnmt3b过表达对大鼠髁突FCSCs成脂、成骨、成软骨相关基因表达的影响.结果:大鼠髁突纤维软骨干细胞具有间充质干细胞特性.与对照组相比,Dnmt3b过表达组成脂相关基因过氧化物酶体增殖物激活受体γ(peroxisome proliferator-activated receptorγ,Ppar-γ)的表达显著降低,成骨相关基因 Runt 相关转录因子 2(runt-related transcription factor 2,Runx2)、碱性磷酸酶(alkaline phosphatase,ALP)的表达显著升高,成软骨相关基因SRY相关转录因子 9(SRY-related high mobility group-box gene 9,Sox9)、聚集蛋白聚糖(aggrecan,AC AN)的表达显著升高.结论:Dnmt3b能促进大鼠髁突纤维软骨干细胞成软骨向分化和成骨向分化,抑制其成脂向分化.
Experimental Study on Regulation of Dnmt3b on Multidirectional Differentiation of Condylar Fibrocartilage Stem Cells
Objective:To investigate the effect of DNA methyltransferase 3b enzyme(Dnmt3b)on adipogenic,os-teogenic,and chondrogenic differentiation of rat condylar fibrocartilage stem cells(FCSCs).Methods:Rat condylar FCSCs were isolated and cultured in vitro.After using lentivirus to overexpress Dnmt3b in FCSCs,the cells were induced by adipogenesis,osteogenesis,and chondrogenesis.The expression of related genes to adipogenesis,osteo-genesis,and chondrogenesis were detected.Results:Rat condylar FCSCs had the characteristics of mesenchymal stem cells.Compared with the control group,the expression of adipogenic gene peroxisome proliferator-activated receptorγ(Ppar-γ)in Dnmt3b overexpression group was decreased significantly,the expression of osteogenic gene Runt-related transcription factor 2(Runx2)and alkaline phosphatase(ALP)was increased significantly,and the ex-pression of chondrogenic gene SRY-related high mobility group-box gene 9(Sox9)and aggrecan(ACAN)was in-creased significantly.Conclusion:Dnmt3b can promote chondrogenic and osteogenic differentiation of rat condylar FCSCs and inhibit their adipogenic differentiation.

DNA methyltransferase 3b enzymecondyle fibrocartilage stem cellsadipogenic differentiationosteogenic differentiationchondrogenic differentiation

杨慧、苏俭生、周玥

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同济大学口腔医学院·同济大学附属口腔医院口腔修复科 上海 200072

上海牙组织修复与再生工程技术研究中心·同济大学口腔医学研究所 上海 200072

同济大学口腔医学院·同济大学附属口腔医院牙体牙髓病科 上海 200072

DNA甲基转移酶3b 髁突纤维软骨干细胞 成脂分化 成骨分化 成软骨分化

国家自然科学基金青年科学基金上海市卫生健康委卫生行业临床研究专项青年基金

8200106920194Y0438

2024

10.13701/j.cnki.kqyxyj.2024.06.006

口腔医学研究
武汉大学口腔医学院

口腔医学研究

CSTPCD北大核心
影响因子：0.48
ISSN：1671-7651
年,卷(期)：2024.40(6)
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