Objective:To investigate the effect of DNA methyltransferase 3b enzyme(Dnmt3b)on adipogenic,os-teogenic,and chondrogenic differentiation of rat condylar fibrocartilage stem cells(FCSCs).Methods:Rat condylar FCSCs were isolated and cultured in vitro.After using lentivirus to overexpress Dnmt3b in FCSCs,the cells were induced by adipogenesis,osteogenesis,and chondrogenesis.The expression of related genes to adipogenesis,osteo-genesis,and chondrogenesis were detected.Results:Rat condylar FCSCs had the characteristics of mesenchymal stem cells.Compared with the control group,the expression of adipogenic gene peroxisome proliferator-activated receptorγ(Ppar-γ)in Dnmt3b overexpression group was decreased significantly,the expression of osteogenic gene Runt-related transcription factor 2(Runx2)and alkaline phosphatase(ALP)was increased significantly,and the ex-pression of chondrogenic gene SRY-related high mobility group-box gene 9(Sox9)and aggrecan(ACAN)was in-creased significantly.Conclusion:Dnmt3b can promote chondrogenic and osteogenic differentiation of rat condylar FCSCs and inhibit their adipogenic differentiation.
关键词
DNA甲基转移酶3b/髁突纤维软骨干细胞/成脂分化/成骨分化/成软骨分化
Key words
DNA methyltransferase 3b enzyme/condyle fibrocartilage stem cells/adipogenic differentiation/osteogenic differentiation/chondrogenic differentiation
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