Effect of Endogenous Hyaluronan on Proliferation of Mouse Dental Papilla Cells
Objective:To explore the effect of endogenous hyaluronan(HA)remodeling on the proliferation of mouse dental papilla cells(mDPCs).Methods:Fast-proliferating cells were labeled with EdU by intraperitoneal in-jection into mice at postnatal day 2.5.mDPCs were isolated,and then treated with different concentrations of hyal-uronidase(HAase).The cell proliferation was detected by CCK-8 assay and EdU assay.Flow cytometry was used to detect cell cycle distribution and apoptosis.The transfection of siHyal2 caused HA accumulation and the cell proliferation was detected by EdU assay.Results:HA expression was mainly observed in the slow-proliferating mesenchymal cells in mouse incisors,while the expression of HA was decreased in transit-amplifying cells(TACs).The CCK-8 results indicated that 800 μg/mL HAase significantly promoted cell proliferation compared with the con-trol group(P＜0.0001).After 48 h of HAase treatment,the EdU assay and flow cytometry results showed an in-crease in cellular DNA synthesis and a significant increase in the ratio of S-phase cells(P＜0.01).Additionally,early cell apoptosis was inhibited(P＜0.05).In contrast,the accumulation of HA around mDPCs was found to sig-nificantly inhibit cell proliferation(P＜0.01).Conclusion:The proliferation of mDPCs is closely related to endoge-nous HA.The degradation of HA promotes cell proliferation,which is inhibited by local HA accumulation.
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