Effects of Platelet-rich Fibrin on Biological Function of Gingival Fibroblasts
Objective:To explore the effects of freeze-dried platelet-rich fibrin(FD-PRF)on human gingival fibro-blasts(hGFs)and antibacterial property of FD-PRF,and to provide a new way for controlling refractory oral mu-cositis(OM).Methods:Cell counting kit-8(CCK-8),flow cytometry,scratch wound,and Transwell assays were used to investigate the effects of FD-PRF on hGFs'cycle,proliferation,and migration.Pour plate and shaking cul-ture methods were explored to investigate the antibacterial ability of FD-PRF.Results:CCK-8 assay showed that hGFs number increased significantly with the increase of FD-PRF concentration.In cell cycle results,proportions of cells at S-G2/M phase:5％FD-PRF group＞1％FD-PRF group＞control group.And proportions of cells at G1-G0 phase were the opposite.Cells migration assays suggested that FD-PRF improved migration rate of hGFs.For anti-bacterial function tests of FD-PRF,it was found that clones of E.coli and S.aureus were both reduced in the FD-PRF group.Conclusion:By facilitating hGFs entering the division stage,FD-PRF promotes proliferation of hGFs.FD-PRF possesses significant chemotaxis toward hGFs.Besides,FD-PRF possesses intrinsic antibacterial property.FD-PRF may promote the healing of oral mucositis.
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