目的:探究局部注射抗核因子κB受体活化因子配体(receptor activator of nuclear factor kappa-B ligand,RANKL)抗体对大鼠牙槽骨骨质疏松的影响及不同剂量间的比较研究.方法:采用SD大鼠30只(对照组9只,实验组21只),通过皮下注射地塞米松建立大鼠牙槽骨骨质疏松模型,建模5周后进行模型验证,模型验证成功后,将实验组随机分为3组;实验组Ⅱ、Ⅲ于1、3、7 d在大鼠右上第一磨牙腭侧黏骨膜下注射兔抗大鼠RANKL抗体0.1 μg/位点和1 μg/位点,实验组Ⅰ注射等体积生理盐水.1周后处死取材,采用微计算机断层扫描技术(micro computed tomography,Micro-CT)测量右上第一磨牙区牙槽骨骨密度(bone mineral density,BMD)、骨体积分数(bone volume fraction,BV/TV);牙槽骨组织病理学染色观察组织形态学改变及破骨细胞数目变化;酶联免疫吸附试验(enzyme linked immunosorbent assay,ELISA)检测牙龈中 RANKL/骨保护素(osteoprotegerin,OPG)比值变化.结果:成功建立大鼠牙槽骨骨质疏松模型.Micro-CT和组织病理学结果显示,注射RANKL抗体后,牙槽骨BMD、BV/TV增加,破骨细胞数目减少,成骨细胞活跃,骨髓腔减小,但高、低剂量抗体相比变化不显著;ELISA结果显示,注射高剂量抗体后牙龈中RANKL/OPG比值显著降低,注射低剂量抗体后RANKL/OPG比值虽降低,但变化不显著.结论:局部注射抗RANKL抗体可以改善大鼠牙槽骨骨质疏松,低剂量即可达到改善效果.
Effects of Local Injection of Different Doses of Anti-RANKL Antibody on Alveolar Bone Osteoporosis in Rats
Objective:To explore the effect of local injection of different doses of anti-receptor activator of nuclear factor kappa-B ligand(RANKL)antibody on alveolar bone osteoporosis in rats.Methods:A total of 30 SD rats were selected,with 9 in the control group and 21 in the experimental group.The rat alveolar bone osteoporosis model was established by subcutaneous injection of dexamethasone.The model was verified 5 weeks later.After successful verification,the experimental group was randomly divided into 3 groups.Experimental group Ⅱ and Ⅲ were injec-ted with 0.1 μg/site and 1 μg/site of rabbit anti-rat RANKL antibody in the palatine side of the upper right first molar of rats on day 1,3,and 7,and experimental group Ⅰ was injected with equal volume of saline.Bone mineral density(BMD)and bone volume fraction(BV/TV)of alveolar bone in the right upper first molar region were meas-ured by micro-CT after 1 week.The changes of histomorphology and the number of osteoclasts were observed by histopathological staining of alveolar bone.En-zyme linked immunosorbent assay(ELISA)was used to de-tect the RANKL/osteoprotegerin(OPG)ratio in gingival tissue.Results:The rat alveolar bone osteoporosis model was established successfully.Micro-CT and histopathology showed that after antibody injection,alveolar bone BMD and BV/TV were increased,the number of osteoclasts were de-creased,osteoblasts were actived,and bone marrow cavity were decreased,but there was no significant change between high and low doses of antibody.ELISA results showed that the ratio of RANK,/OPG in gingival was significantly decreased after high dose antibody injection,however,it was decreased without significance after low dose antibody injection.Conclusion:Local injection of anti-RANKL an-tibody can improve the alveolar bone osteoporosis in rats,and low dose could reach the improvement effect.
nuclear factor κB receptor activator ligand inhibitoranti-receptor activator of nuclear factor-κB lig-and antibodyreceptor activator of nuclear factor-κB ligandosteoprotegerinalveolar bone osteoporosis
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