Objective:To establish and analyze a mouse model of syndromic cleft lip and palate in mice.Methods:Pregnant mice on gestation day 10.5(GD10.5)were gavaged with 90 mg/kg of atRA or corn oil and DMSO sol-vent.Upon euthanasia of pregnant mice on GD17.5,embryos were collected.Stereomicroscope,paraffin embed-ding and sectioning,and hematoxylin-eosin(HE)staining were used to observe the development of embryos.Immunofluorescence staining was used to verify the expres-sion of retinoic acid receptor alpha(RARα).Results:The embryos in the atRA group showed cleft palate,limb de-formities,abnormal molar development,and non-compac-tion of ventricular myocardium.Immunofluorescence stai-ning showed the expression of RARα increased in molar and palate of embryos of the atRA group.Conclusion:The mouse cleft palate model induced by atRA presents as syn-dromic cleft lip and palate,and atRA affects the develop-ment of mice by activating of RARα receptor.
关键词
全反式维甲酸/综合征型唇腭裂/先天性心脏病/磨牙/成釉细胞
Key words
all-trans retinoic acid/syndromic cleft lip and palate/congenital heart defects/molar/ameloblasts
维普
万方数据