目的:对不同患龋儿童唾液进行高通量测序并分析低龄儿童龋(early childhood caries,ECC)的影响因素,为探究ECC与菌群的相关性及ECC综合诊断模型的建立提供依据.方法:对儿童口腔检查,根据结果分为无龋(caries free,CF)组、ECC组和重度低龄儿童龋(severe early childhood caries,SECC)组,采取问卷调查方法探究ECC的影响因素;同时对儿童唾液进行高通量测序,并拟采用受试者工作曲线(receiver operating characteristic curve,ROC)建立基于以上结果的疾病诊断模型.结果:不同患龋儿童的微生物群落组成存在显著统计学差异(P＜0.05).在前15个细菌属中,韦荣菌属(Veillonella)、纤毛菌属(Leptotrichia)、拟普雷沃菌属(Alloprevotella)丰度水平的差异在3组间具有统计学意义(P＜0.05).Veillonella和Leptotrichia为ECC组的标志物种,Alloprevotella在SECC组中显著富集.戒断奶瓶喂养的年龄、Veillonella、Leptotrichia、Alloprevotella联合应用筛选高患龋风险儿童的ROC曲线下面积为0.754.结论:Veillonella、Leptotrichia、Alloprevotella可能为患龋儿童唾液中潜在的致龋菌.戒断奶瓶喂养的年龄、Veillonella、Leptotrichia、Alloprevotella联合检测对筛选高患龋儿童具有诊断价值.
Study on Saliva Microbial Community and Construction of Prediction Model for Young Children with Caries
Objective:To analyze the influencing factors of early childhood caries(ECC)and to provide a basis for exploring the correlation between ECC and flora and establishing a comprehensive diagnostic model of ECC through high-throughput sequencing on saliva of children with different caries status.Methods:Children were examined oral-ly and divided into three groups according to the results:Caries free(CF)group,ECC group,and Severe early childhood caries(SECC)group.The influencing factors of ECC were investigated by questionnaire.At the same time,saliva of three groups of children was collected for high-throughput sequencing,and a disease diagnosis model based on the above results was established by receiver operating characteristic curve(ROC).Results:There was significant statistical difference in the composition of microbial communities among groups with different caries sta-tus(P＜0.05).Among the top 15 bacterial genera,the abundance levels of Veillonella,Leptotrichia,and Allo-prevotella were significantly different(P＜0.05).Veillonella and Leptotrichia were the marker species of ECC group.Alloprevotella was significantly enriched in SECC group.The area under the ROC curve for screening chil-dren with high caries risk was 0.754,which was based on the age of weaning from bottle feeding,the combined ap-plication of Veillonella,Leptotrichia,and Alloprevotella.Conclusion:Veillonella,Leptotrichia,and Alloprevo-tella may be potential cariogenic bacteria in saliva of children with caries.The age of weaning from bottle feeding,the combined application of Veillonella,Leptotrichia,and Alloprevotella are of diagnostic value for screening chil-dren with high caries.
early childhood cariesmicrobial communityprediction modelhigh-throughput sequen-cing
万方数据
维普
