Study on Lugol's Iodine Enhanced Micro-CT Imaging for Evaluating Margin Status of Oral Squamous Cell Carcinoma
Objective:To assess the incisal margin status of oral squamous cell carcinoma(OSCC)in three dimen-sions using Lugol's iodine enhanced Micro-CT imaging.Methods:A total of 68 human OSCC in vitro samples were collected for this study.All samples were stained with 3％solution of Lugol's iodine for 12 hours and underwent Micro-CT scanning with a layer thickness of 50 μm.Subsequently,the Lugol's iodine stained samples were subjected to routine hematoxylin-eosin(HE)staining sections(4 μm)to create digital pathological sections.The mucosal margin(MM)and deep margin(DM)were measured separately on both the imaging and pathological images.Results:Lugol's iodine enhanced Micro-CT imaging can effectively differentiate tumor boundaries in three-dimensional space,providing more accurate measurements of MM and DM compared to two-dimensional pathological sections,thus enabling identification of positive incisal margins and critical incisal margins.Conclusion:Lugol's iodine enhanced Micro-CT imaging allows comprehensive evaluation of OSCC incisal margins in three dimensions,offering more comprehensive and clear information regarding tumor margins which can guide clinical treatment plan selection and prognosis assessment.
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