摘要
从41个滩涂湿地样品中分离得到45株木霉菌株，并对得到的木霉菌株进行了平板耐盐实验和黄瓜种子发芽实验。结果表明，PDA平板中NaCl浓度为0．4 mol／L时，供试菌株的耐盐率范围在17．9％～85．5％之间，大部分菌株的耐盐率在50．0％～80．0％之间，不同菌株的耐盐率存在显著性差异（ p＜0．01），其中哈茨木霉TW20015和TW20239耐盐率最高，分别达到了81．6％和85．5％。通过黄瓜种子发芽实验表明，种子发芽率随NaCl浓度的提高而降低；加入TW20015、TW20239后可以提高种子的耐盐阈值。在NaCl浓度为20 mg／mL时仍有种子萌发，而对照组NaCl浓度为16 mg／mL时已无种子萌发。2株木霉均提高了黄瓜种子的发芽率和芽长，其中TW20015效果更好。本实验为进一步应用木霉提高植物的耐盐能力奠定了基础。
Abstract
45 Trichoderma isolates were obtained from 41 tidal flat wetland samples, determined the salt tolerance and cucumber germination rate with Trichoderma isolates involved.The result indicated that when adding 0.4 mol/L NaCl solution in PDA, the salt tolerance rate had range from 17.9%to 85.5%with all test strains, and the salt toler-ance rate had range from 50.0%to 80.0%with most test strains.By Duncan’s analysis of the data was made using the statistical program SPSS10.0 for Windows, significant difference was indicated at 0.01 level between different strains, T.harzianum TW20015 and TW20239 had the best efficiency with 81.6%and 85.5%among them.Through the cucumber seed germination test, as the NaCl concentration increased, seed germination ability was inhibited.The salt-tolerant threshold of cucumber seeds could enhanced to 20 mg/mL NaCl by adding TW20015 or TW20239, on the other hand, seeds didn’ t germinate at 16 mg/mL NaCl without Trichoderma isolates.TW20015 and TW20239 could enhanced germination rate and germinal length compare with blank control, TW20015 had most efficiency especially. This test provide the theoretical basis for its development and Trichoderma utilization in saline areas.
NETL
NSTL
维普
万方数据