In order to effectively repair and beautify the polluted soil and reduce the pollution of heavy metals,the cultivation of bi-ological agents and two enrichment plants were combined to repair the lead soil.After 30 and 60 days of combined remediation with 400 mg/kg,the concentration of heavy metals in soil was decreased 19.61%and 19.48%,respectively.So the adsorption rate were increased by 16.82%and 17.83%,respectively.The activity of catalase was increased from 5.6 mg/kg to 11.4 mg/kg and 15.9 mg/kg.The activity of alkaline phosphatase was increased from 45.6 mg/kg to 88.4 mg/kg and 93.4 mg/kg,respectively.The activity of su-crase was increased from 4.2 mg/kg to 9.6 mg/kg and 9.9 mg/kg,respectively.So the activity of urease was increased from 32.6 mg/kg to 66.3 mg/kg and 71.2 mg/kg,respectively.The content of chlorophyll was increased 9.16%and 9.18%,respectively.The num-ber of heavy metal resistant bacteria was decreased 21.31%and 20.27%,respectively.The number of bacteria,fungi and actinomycetes were increased by 97.68%,67.63%and 210.09%,respectively.The diversity and abundance of microbial community were increased 2.7%and 2.9%,respectively.The results showed that the photosynthesis of plants were enhanced.The adsorption and transport of heavy metals were accelerated.So were the enzyme activity,and the tolerance of plants to heavy metals were enhanced gradually.Mean-time,the number of heavy metal-resistant bacteria were decreased apparently.And so on,the number of bacteria,fungi and actinomyce-tes were increased significantly.It was concluded that the plants and microbial agents could effectively promote the adsorption of plants to heavy metals and strengthen the repair effect,and which could provided scientific basis for the study of heavy metal pollution remediation.
关键词
污染土壤/重金属/生态修复/重金属抗性菌/微生物多样性
Key words
contaminated soil/heavy metal/biological restoration/heavy metal resistant bacteria/microbial community structure