Respiratory Metabolomics of Radiation-damaged Rats Based on GC-MS
To explore the effects of radiation injury on metabolic pathways by detecting the volatile organic compounds(VOCs)in exhaled breath of rats with radiation-induced lung injury based on gas chromatography-mass spectrometry(GC-MS),and to establish a non-invasive,convenient and rapid method for the diagnosis of radiation injury.In response to the characteristics of the radiation injury model,a rat radiation lung injury model was established using gradient dose irradiation with a cobalt source,and the model rats were randomly divided into four groups,one blank group(0 Gy)and one irradiation group each of 2,4 and 6 Gy.The exhaled breath were collected at 1,3,5,7 days after irradiation and analyzed by GC-MS.The samples were analyzed by principal component analysis(PCA)and orthogonal partial least squares-discriminant analysis(OPLS-DA)to find the differential metabolites of relevant VIP＞1,and further explore the potential markers of radiation-induced lung injury.The results show that there are significant differences in blood phase indexes,HE stained sections of lung tissue and VOCs in exhaled breath between the blank and irradiation groups.25 kinds of metabolites are initially screened,among which 5 kinds could be used as combinative potential biomarkers.The construction of this discriminant model provides a theoretical basis for the respiratory histology of radiation injury and an experimental reference for the establishment of a diagnostic method for the non-invasive detection of radiation injury.
万方数据
维普
