Anaerobic ammonia oxidizing bacteria(AnAOB)have poor retention capacity in actual denitrification processes,which is one of the main technical bottlenecks restricting the large-scale application of this technology.Compared with traditional biological fillers,high-temperature pyrolysis tires can be used to prepare a new type of biological filler with conductive properties.This not only recycles fixed waste,but also achieves a dual effect of accelerating the formation of biofilm in AnAOB and improving denitrification effi-ciency.The experimental results indicate that 800 ℃ is the most suitable for pyrolysis tire fillers,while having higher electrical con-ductivity and more favorable characteristics for fixed microbial growth.Compared to the Anammox group alone,the average removal rates of NH4+-N,NO2--N,and TN with Anammox+conductive filler increased by 9.12％,13.20％,and 15.69％,respectively,compared to the Anammox group alone,and were significantly higher than the strengthening effect of polypropylene(PP)filler.The Q-PCR analysis results showed that adding conductive fillers can promote an increase in the abundance of denitrifying bacteria,with AnAOB,nitrite re-ducing bacteria,and nitrate reducing bacteria increasing by 50.21％,69.70％,and 145.59％,respectively.The development of this new conductive filler technology can provide new ideas and data basis for the promotion of Anammox technology.
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