In Vitro Release of Copper-loaded Nanoparticles from Nasal in situ Gels
In order to study the in vitro release pattern of copper tri ethylenetetramine(TETA-Cu)from copper-loaded nanoparticles in nasal in situ gels(TETA-Cu-NPs-gel).Dialysis was used to investigate in vitro release.0.1 mol/mL phosphate buffer solution(PBS,pH=7.4)was used as the release medium and the model was fitted to the release data using Origin software.HPLC(high per-formance liquid chromatography)was used to investigate the release level of TETA-Cu in dissolution media.The chromatographic col-umn was Swell ChromplusTM C18(4.6 mm × 150 mm,5 µm),mobile phase A was pH=3.0 phosphate buffer,and mobile phase B was methanol(A:B=90:10).The flow rate was 1.0 mL/min,the detection wavelength was 260 nm,Intake quantity 20 μL.The results show that TETA-Cu showed good linearity in the range of 0.05～1 mg/mL.The recoveries of the high,medium,and low con-centrations are in the range of 99.9％～101.0％,with the RSDs of less than 2％.Compared with the TETA-Cu solution,the rate of drug release of TETA-Cu-NPs-gel is slow,which conformed to the kinetic equation of the first level of drug release.It is concluded that the in vitro release assay of TETA-Cu-NPs-gel established in this study is accurate,simple,and feasible.TETA-Cu-NPs-gel has a slow-release effect.
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