Ciprofol can alleviate renal ischemia-reperfusion injury in rats via ferroptosis SystemXc-/GPX4 pathway
Objective To investigate the protective effect of Ciprofol on renal ischemia-reperfusion injury and its mechanism.Methods The established renal ischemia-reperfusion model of SD rats was intervened by several drugs besides Ciprofol,ferrostatin-1(inhibitor of ferroptosis)and Erastin(agonist of ferroptosis)alone or in combination.After 24 hours,the rats were sacrificed under anesthesia,and their heart blood taken to detect creatinine and urea nitrogen to evaluate the renal function.At the same time,the kidneys of rats were taken,part of which stained with hematoxylin-eosin staining and the paller score was used to evaluate the degree of renal pathological damage in rats.The other part was used to detect the level of ferroptosis-related indicators of reactive oxygen species,glutathione,ferrous iron,malondialdehyde,the expression and tran-scription level of SLC7A11,the main subunit of SystemXc-,and the downstream functional protein GPX4.Results Compared with the control group,the levels of creatinine,urea nitrogen,paller score,reactive oxygen speciesferrous iron,malondialdehyde in the Ciprofol and Fer-1 group were lower(P<0.05).Compared with the control group,the levels of glutathione,SLC7A11,GPX4 in the Ciprofol group were higher;besides,the expression levels of SLC7A11 and GPX4 related genes were higher in the Ciprofol group(P<0.05).Com-pared with the Ciprofol group,the levels of glutathione,SLC7A11,GPX4 and the expression levels of SLC7A11 and GPX4 related genes were lower in the Ciprofol+Erastin group(P<0.05).Conclusion Ciprofol can reduce renal ischemia-reperfusion injury to protect the kidney,and the mechanism is related to the System-Xc-/GPX4 pathway.
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