一种新型二氧化硅多孔材料固定化酶光纤葡萄糖传感器的研究
A novel glucose optical fiber biosensor with enzyme immobilized by SiO2 porous material
姚润麒 1张鹏飞 2高泽林 1姜兴东 1孟文勃 3丁莉芸4
作者信息
- 1. 兰州大学物理科学与技术学院,甘肃 兰州 730000
- 2. 武汉理工大学国家光纤传感技术与网络工程研究中心,湖北 武汉 430070
- 3. 兰州大学第一临床医学院,甘肃 兰州 730000
- 4. 兰州大学物理科学与技术学院,甘肃 兰州 730000;武汉理工大学国家光纤传感技术与网络工程研究中心,湖北 武汉 430070
- 折叠
摘要
目的 研制一种新型的基于氧敏感指示剂Ru(bpy)3Cl2和固定化葡萄糖氧化酶(GOD)的复合敏感膜光纤传感器,用于实时检测葡萄糖溶液质量浓度.方法 以多孔SiO2纳米粒子为载体固定GOD,并采用溶胶-凝胶法同时加入Ru(bpy)3Cl2制备葡萄糖敏感膜,通过提拉法在光纤端面沉积敏感膜制成光纤传感探头,搭建光纤传感器用于实时检测葡萄糖溶液质量浓度.结果 光纤传感器检测葡萄糖溶液质量浓度为50～900 mg/dL(2.78～50.00 mmol/L),在用于实时监测葡萄糖溶液质量浓度变化时响应时间短,测试标准曲线线性良好.结论 SiO2多孔材料固定化酶光纤葡萄糖传感器具有灵敏度高、响应速度快、检测范围大等优点,未来可以检测人体血液中的葡萄糖质量浓度.
Abstract
Objective To develop an optical fiber sensor with a composite sensitive film containing aerobic sensitive indicator Ru(bpy)3 Cl2 and immobilized glucose oxidase(GOD)for glucose detection.Methods In this work,an enzyme-based optical fiber fluorescence biosensor was prepared by using the SiO2 nanoparticles immobilized with GOD(SiO2-GOD),where the fluorescence indicator was Ru(bpy)3 Cl2.The pull-up method was used to deposit a sensitive film on the face of an optical fiber to produce an optical fiber sensing probe,and then optical fiber sensor was constructed to detect the different concentrations of the glucose solution.Results The optical fiber sensor detected glucose solution concentration in the range of 50-900 mg/dL(2.78～50.00 mmol/L),which could be used for the real-time monitoring of the glucose concentration changes.The response time of the sensor was short,and the testing standard curve had good linearity.Conclusion The fiber optic glucose sensor has the advantages of fast response,high sensitivity and wide detection range for glucose.In the future,it can be used to detect glucose concentration in human blood.
关键词
葡萄糖检测/二氧化硅多孔材料固定化酶/荧光信号/光纤传感器Key words
glucose detection/immobilization of enzymes using porous silica materials/fluorescence signal/optical fiber sensor引用本文复制引用
基金项目
国家自然科学基金面上项目(51878524)
湖北省自然科学基金杰出青年人才项目(2021CFA041)
出版年
2024
国家科技期刊平台
NSTL
万方数据