Protective role of triptolide combined with pulsed radiofre-quency in anti-inflammatory and analgesic effects via Wnt/β-catenin signaling pathway
Objective To investigate the anti-inflammatory and analgesic effects of triptolide combined with pulsed radiofrequency(RF)on neuropathic pain in rat models and related molecular mechanisms.Methods SD rats were randomly divided into a sham surgery group,spinal cord nerve ligation group,trip-tolide group,pulse RF group and triptolide combined pulse RF group.Spinal cord nerve ligation-model rats were cultivated and pain behavior changes in them measured.The expressions of Wnt-3α,β-catenin and friz-zled7 in sciatic nerve tissue were measured by Western blotting,mRNA expression of Il-1β,Cox-2,Tnf-α and p65 Nf-κB in sciatic nerve tissue measured by polymerase chain reaction and expression of SP and C-fos protein in sciatic nerve tissue detected by immunohistochemistry.Results Except for the sham surgery group,the mechanical foot contraction reflex threshold of the other groups sharply decreased on the first day after surgery;on the fifth day,the mechanical foot contraction reflex threshold of the spinal cord nerve ligation group tended to stabilize,while that of each treatment group gradually increased.Except the sham surgery group,the thermal shrinkage reflex latency of the other groups decreased sharply on the first 3 days after sur-gery,and began to increase on the third day,and the rate of increase in all treatment groups was higher than that in the spinal cord nerve ligation group.The results of the protein immunoblotting showed that,on postop-erative day 3,7 and 14,the expression of Wnt-3α,β-catenin,and frizzled7 proteins in the spinal cord nerve ligation group was higher compared to the sham surgery group.Compared to the spinal cord nerve ligation group,the differences in the expression of Wnt-3α,β-catenin,and frizzled7 proteins between the treatment groups were relatively small on postoperative day 3,but decreased in all treatment groups on pday 7 and 14,with the combined treatment of triptolide and pulse radiofrequency group showing the lowest relative expres-sion level.The results of polymerase chain reaction analysis revealed an increase in the expression of Il-1β,Cox-2,Tnf-α,and p65 Nf-κB mRNA in the spinal cord nerve ligation group compared to the sham surgery group on postoperative days 3,7 and 14.Compared to the spinal cord nerve ligation group,the mRNA expres-sion in all treatment groups decreased on postoperative days 7 and 14,with the combination of triptolide and pulsed radiofrequency group showing the lowest expression level.The immunohistochemical results showed that the protein expression of SP and C-fos in the spinal cord nerve ligation group was higher than that in the sham surgery group,and the expression of the two proteins increased gradually with the extension of time.Compared with the spinal cord nerve ligation group,the protein expression levels of SP and C-fos in all treatment groups decreased with the prolongation of treatment time,and the decrease was most obvious in triptolide combined pulse RF group.Conclusion Triptolide combined with pulsed radiofrequency treatment alleviates inflammation and pain response in a rat model of neuropathic pain by regulating the Wnt/β-catenin signaling pathway.
triptolidepulse radio frequencyneuropathic painWnt/β-catenin signaling pathwayinflammation
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