Objective To explore the inhibitory effects of the Chinese medicinal therapy of"regulating the liver and tonifying the kidney"on breast cancer and to preliminarily study its mechanisms.Methods The network pharmacology method was used to predict the mechanisms of the therapy in"regulating the liver and tonifying the kidney";the Cell Counting Kit-8,single-cell cloning and scratch assays were used to detect the effects of the Chinese medicines on the proliferation,clone formation,and migration abilities of human breast cancer cells MCF-7 and T47D-Western blotting was used to detect the expression of phosphorylated phos-phoinositide 3-kinase(PI3K)and Akt kinase proteins in the Chinese medicines.Results Network harmacology screened out 84 co-targets of"active components-breast cancer",and the mechanisms were related to signal-ing pathways such as PI3K/Akt and mitogen-activated protein kinase.In vitro experiments showed that the Chinese medicines could inhibit the proliferation and migration of MCF7 and T47D cells,and reduce the expression levels of PI3K and Akt kinase proteins.Conclusion The"regulating the liver and toni-fying the kidney"therapy can be used to treat breast cancer,may inhibit the proliferation and invasion of hu-man breast cancer MCF-7 and T47D cells by regulating the PI3K/Akt signaling pathway.
关键词
乳腺癌/网络药理学/调肝补肾/增殖抑制/磷脂酰肌醇3激酶/Akt信号通路
Key words
breast cancer/network pharmacology/regulating the liver and tonifying the kidney/proliferation inhibition/phosphoinositide 3-kinase/Akt signaling pathway
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