Application of an infrared-based odorized hole-board test instrument in the study of olfactory function in mice
Objective To establish experimental methods of olfactory discrimination,recognition,spatial memory and preference in mice by using sensitive infrared sensors instead of capacitive sensors and using them in the odorized hole-board test instrument in order to test the stability of the olfactory functions of mice studied by the instrument.Methods Habituation/dishabituation test,olfactory spatial memory test,propionic acid olfactory threshold test and olfactory preference test were used to evaluate the olfactory function of normal C57 BL/6J mice.Results C57 BL/6J mice were capable of olfactory discrimination and spatial memo-ry of olfactory cue association.The olfactory threshold for propionic acid was 10-3 kPa,and the preference for geraniol the highest,while the preference for L-carvone the lowest.Conclusion The infrared-based odor-ized hole-board test instrument used in this study can satisfy the requirements of olfactory behavior experi-ments such as discrimination,recognition,learning and memory,and preference of mice.
olfactionolfactory functionolfactory behavioral experimentodorized test instrumentinfrared sensorsmice
万方数据
维普
