Objective To establish experimental methods of olfactory discrimination,recognition,spatial memory and preference in mice by using sensitive infrared sensors instead of capacitive sensors and using them in the odorized hole-board test instrument in order to test the stability of the olfactory functions of mice studied by the instrument.Methods Habituation/dishabituation test,olfactory spatial memory test,propionic acid olfactory threshold test and olfactory preference test were used to evaluate the olfactory function of normal C57 BL/6J mice.Results C57 BL/6J mice were capable of olfactory discrimination and spatial memo-ry of olfactory cue association.The olfactory threshold for propionic acid was 10-3 kPa,and the preference for geraniol the highest,while the preference for L-carvone the lowest.Conclusion The infrared-based odor-ized hole-board test instrument used in this study can satisfy the requirements of olfactory behavior experi-ments such as discrimination,recognition,learning and memory,and preference of mice.
关键词
嗅觉/嗅觉功能/嗅觉行为学/嗅觉测试仪/红外传感器/小鼠
Key words
olfaction/olfactory function/olfactory behavioral experiment/odorized test instrument/infrared sensors/mice
维普
万方数据