分级醇沉败酱草多糖的理化特征及抑制糖消化酶活性研究
Physicochemical characteristics of graded alcohol precipitation Herba Patriniapolisa Calid and inhibition of sugar-digesting enzyme activity
魏赛雪 1惠和平 2郭栋费 1张心驰 1郭敏 3李晓东3
作者信息
- 1. 甘肃中医药大学 药学院,甘肃 兰州 730000
- 2. 商洛学院 生物医药与食品工程学院,陕西 商洛 726000
- 3. 甘肃中医药大学 药学院,甘肃 兰州 730000;甘肃省中医院 中药研究所,甘肃 兰州 730050;甘肃省中医药研究院 陇药大品种二次开发及临床疗效评价行业技术中心,甘肃 兰州 730050
- 折叠
摘要
目的 探究不同分级败酱草多糖的理化特征、糖消化酶抑制活性以及两者的相关性.方法 采用热水提取、不同浓度乙醇(50%、60%、70%、80%)醇沉得到4种败酱草多糖HP-50、HP-60、HP-70和HP-80,使用苯酚-硫酸法、气相色谱、高效凝胶渗透色谱和紫外分光度法分别测定多糖的含量、单糖组成、分子量等理化特征以及对α-淀粉酶和α-葡萄糖苷酶抑制活性,运用双变量相关性分析比较4种多糖抑制糖消化酶的活性与其理化特征的相关性.结果 所有败酱草多糖均表现出浓度依赖性的糖消化酶抑制活性,其中HP-80的糖消化酶抑制活性最好;与其他醇沉浓度相比,HP-80葡萄糖摩尔分数较高,鼠李糖和阿拉伯糖摩尔分数较低,相对分子量较小.结论 败酱草多糖相对分子量为50～100 kDa、鼠李糖摩尔分数在9.83%以下、阿拉伯糖与葡萄糖物质的量比小于1更有利于败酱草多糖抑制α-淀粉酶和α-葡萄糖苷酶的活性.
Abstract
Objective To investigate the physicochemical characteristics and glycodigestive enzyme inhibitory activity of different fractions of Herba patriniae polysaccharides,as well as the correlation between them.Methods Four kinds of HPs(HP-50,HP-60,HP-70 and HP-80)were obtained by hot water extraction and alcohol precipitation at different concentrations of ethanol(50%,60%,70%,80%),and the content and mono-saccharide composition of polysaccharides determined by phenol-sulfuric acid method,gas chromatography(GC),high performance liquid chromatography(GPC)and ultraviolet spectrophotometry(UV).The activities of four polysaccharides in inhibiting sugar-digesting enzymes were compared via a bivariate correlation analy-sis regarding their physical and chemical characteristics such as molecular weight and inhibitory activity against α-amylase and α-glucosidase.Results All HPs exhibited concentration-dependent inhibition of sugar-digesting enzymes,and HP-80 had the best inhibitory activity of sugar-digesting enzymes.Compared with oth-er alcohol precipitation concentrations,HP-80 had a higher glucose content,rhamnose and arabinose content was lower,and the molecular weight smaller.Conclusion It is speculated that the relative molecular weight of Herba patriniae polysaccharides is 50～100 kDa,the molar fraction of rhamnose less than 9.83%and the amount ratio of arabinose to glucose substance less than 1,which could more conducive to the inhibition of α-amylase and α-glucoside activities of HPs.
关键词
分级醇沉/败酱草多糖/α-淀粉酶抑制活性/α-葡萄糖苷酶抑制活性/相关性分析Key words
graded alcohol sinking/Herba Patriniae polysaccharide/α-amylase inhibitory activity/α-glucosi dase inhibitory activity/correlation analysis引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据