Endothelial cells and fibroblasts mediate the microenvironmental regulatory network of carotid body paraganglioma
Objective:To explore the gene expression characteristics of endothelial cells and fibroblasts in the microenvironment of SDHD-mutated carotid body tumors(SDHD-CBT),to fine the functional enrichment of each subcluster,and to further explore the network of cell-cell interactions in the microenvironment of SDHD-CBT.Methods:The bioinformatics analysis was used to download and reanalyze the single-nuclear RNA sequen-cing data of SDHD-CBT,SDHB mutated thoracic and abdominal paraganglioma(SDHB-ATPGL),SDHB-CBT,and normal adrenal medulla(NAM),to clarify the information of cell populations of the samples.We fo-cused on exploring the gene expression profiles of endothelial cells and fibroblasts subclusters,and performed functional enrichment analysis based on Gene Ontology(GO)resources.CellChat was used to compare the cell-cell interactions networks of different clinical samples and predict significant signaling pathways in SDHD-CBT.Re-sults:A total of 7 cell populations were profiled.The main subtypes of endothelial cells in SDHD-CBT are arterial and venous endothelial cells,and the main subtypes of fibroblasts are myofibroblasts and pericytes.Compared to NAM,SDHB-CBT and SDHB-ATPGL,cell communication involving endothelial cells and fibroblasts in SDHD-CBT is more abundant,with significant enrichment in pathways such as FGF,PTN,WNT,PROS,PERIOSTIN,and TGFb.Conclusion:Endothelial cells and fibroblasts in SDHD-CBT are heterogeneous and in-volved in important cellular interactionprocesses,in which the discovery of FGF,PTN,WNT,PROS,PERIOSTIN and TGFb signals may play an important role in the regulation of microenvironment of SDHD-CBT.
carotid body tumorssingle-nuclei RNA sequencingendothelial cellsfibroblastscell-cell interactome
NETL
NSTL
万方数据
