Clinical features and serum lipidomic profile of patients with nonalcoholic fatty liver disease and healthy individuals in the overweight population
objective To investigate the differences in clinical indices and lipid metabolism between the patients with nonalcoholic fatty liver disease(NAFLD)and healthy individuals in the overweight population.Methods In this study,body mass index(BMI)>23 kg/m2 was defined as overweight.A total of 62 overweight patients with NAFLD who were admitted to Putuo Hospital Affiliated to Shanghai University of Traditional Chinese Medicine from August 2020 to April 2021 were enrolled as overweight NAFLD group,and 50 overweight individuals who underwent physical examination during the same period of time were enrolled as control group.Clinical information and blood biochemical parameters were recorded for all subjects.Ultra-performance liquid chromatography-tandem mass spectrometry was used to analyze serum lipidomic profile,and principal component analysis and orthogonal partial least squares-discriminant analysis were used to perform the multivariate statistical analysis of lipidomic data.The chi-square test was used for comparison of categorical data between two groups,and the independent-samples t test or the Wilcoxon rank-sum test was used for comparison of continuous data between two groups.Results The overweight NAFLD group had a significantly higher BMI than the overweight control group(Z=-2.365,P=0.018).As for serological markers,compared with the overweight control group,the overweight NAFLD group had significantly higher levels of red blood cells,hemoglobin,hematocrit,uric acid,total protein,globulin,alkaline phosphatase,gamma-glutamyl transpeptidase,alanine aminotransferase,aspartate aminotransferase,cholinesterase,low-density lipoprotein,total cholesterol,triglyceride,apolipoprotein B,and blood glucose(all P<0.05).The lipidomic analysis showed that there was a significant difference in lipid metabolism between the two groups,and a total of 493 differentially expressed lipids were identified(VIP value>1,P<0.05),among which 143 lipids were significantly upregulated and 350 lipids were significantly downregulated in the overweight NAFLD group.The mean total fatty acid content in the overweight NAFLD group was 3.6 times that in the overweight control group.Compared with the overweight control group,the overweight NAFLD group had a significant reduction in the content of triglyceride with>3 unsaturated bonds(P<0.001)and a significant increase in the content of triglyceride with≤3 unsaturated bonds(P<0.001).Conclusion Compared with healthy overweight individuals,overweight NAFLD patients tend to have significant abnormalities in some biochemical parameters and lipid metabolites,with significant increases in the content of fatty acid in blood and the types of saturated fat chains in triglycerides.
Non-Alcoholic Fatty Liver DiseaseOverweightSigns and SymptomsLipidomics
万方数据
维普
