Objective To investigate the effect of robot-assisted percutaneous screwing of injured vertebra for treat-ment of thoracolumbar burst fractures.Methods The 56 patients with thoracolumbar burst fractures were divided into the observation group(28 cases were treated with robot-assisted percutaneous screwing of injured vertebra)and control group(26 cases were treated with screwing of injured vertebra via Wiltse approch),according to different treatment methods.The surgical condition,pain VAS,anterior vertebral height ratio,Cobb angle of the injured vertebrae were re-corded.The accuracy of pedicle screw position based on Gertzbein Robbins criteria was evaluated.Results All pa-tients were followed up for 3~12 months.The observation group had fewer(shorter)intraoperative bleeding volume,intraoperative fluoroscopy frequency,incision length,hospital stay and callus formation time,compared to the control group(P<0.01),and there was no statistical difference in surgical time between the two groups(P>0.05).There were no statistical significance in the excellent-good rate and accuracy rate of screw placement between the two groups(P>0.05).The pain VAS:At 1,3 d after surgery,observation group was lower than that of the control group(P<0.05),and there was no statistical significance between the two groups at 1 and 3 months after surgery(P>0.05).There was no statistical significance in the anterior edge height ratio and Cobb angle of the injured vertebra between two groups immediately and 3 months after surgery(P>0.05).Conclusions Robot-assisted percutaneous screwing of injured vertebra for treatment of thoracolumbar burst fractures can reduce intraoperative bleeding,intraoperative flu-oroscopy frequency,shorten patient hospitalization time,and relieve early postoperative pain.