Objective To investigate the mechanism of miR-148a improving intervertebral disc degeneration(IDD)by regulating Wnt/β-catenin signal pathway.Methods Interleukin(IL)-1 β was inducted to establish the models of IDD rats and cells,in order to observe the influence of miR-148 on the following indicators:Pathological morphology of in-tervertebral disc tissue,intervertebral disc cartilage tissue,viability and apoptosis of intervertebral disc nucleus pulpo-sus cells,levels of IL-6 and IL-18 in serum and intervertebral disc nucleus pulposus cells,expression of protein type Ⅱ collagen(Collagen Ⅱ),proteoglycan(Aggrecan)as mechanistic markers in intervertebral disc tissue and nucleus pulposus cells;expression of miR-148a and Wnt/β-catenin pathway-related proteins(Wnt,β-catenin)in intervertebral disc tissue and nucleus pulposus cells.Results Compared with the sham operation group(rat)/control group(cell),for model group,the intervertebral disc tissue degenerated and cartilage matrix was massively lost,the viability of nu-cleus pulposus cells,the protein expression levels of Collagen Ⅱ and Aggrecan were all decreased(P＜0.05),the ap-optosis rate of nucleus pulposus cells,the levels of IL-6 and IL-18,the protein expression levels of Wnt and β-catenin were all increased(P＜0.05).Compared with the model group,for the miR-148a overexpression group,the interverte-bral disc tissue degeneration and the cartilage matrix loss in rats were alleviated,the viability of nucleus pulposus cells,the protein expression levels of Collagen Ⅱ and Aggrecan were all increased(P＜0.05),the apoptosis rate of nucleus pulposus cells,the levels of IL-6 and IL-18,and the protein expression levels of Wnt and β-catenin were all decreased(P＜0.05).Conclusions miR-148a can inhibit inflammation by down-regulating the expression of Wnt/β-catenin signal pathway,thereby reducing the damage of intervertebral disc and cartilage in IDD rats,relieving carti-lage matrix loss and nucleus pulposus cell apoptosis,and improving the degeneration symptoms of intervertebral disc tissue in rats.
miR-148aWnt/β-catenin signal pathwayintervertebral disc degenerationrats
维普
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