Protective effect of Qingyi Ⅱ preparation on multiple organ functions in rats with MODS caused by wasp venom
Objective To observe the protective effect of Qingyi Ⅱ preparation on multiple organ functions in rats with MODS caused by wasp venom.Methods Thirty-six healthy male SD rats of SPF grade were randomly divided into model group,blank control group,Qingyi Ⅱ group,12 rats in each group.The model group and Qingyi Ⅱ group were injected with wasp venom intravenously to establish the MODS rat model,the blank control group was injected with an equal volume of saline intravenously,and the Qingyi Ⅱ group was given 10 ml/kg of the Qingyi Ⅱ preparation intranasally,whereas the model group and the blank control group were given an equal volume of saline by gavage via nasogastric gavage.After 24 hours and 72 hours of intervention,cardiac,hepatic and renal function indexes(cTnT,ALT,SCr),serum intestinal function indexes(DAO,ETX),serum inflammatory factors(IL-6,IL-8,IL-1 β,TNF-α)levels and the expression of p-Akt/Akt,p65,p-p65 in colorectal tissues of the three groups were compared.Results(1)Cardiac,hepatic,renal and intestinal function indexes and inflammatory factors:compared with the blank control group,the levels of cTnT,ALT,SCr,DAO,ETX,IL-6,IL-8,IL-1β,TNF-α in the rats of the model group were elevated at 24 hours and 72 hours of intervention(P＜0.05).Compared with the model group,the levels of cTnT,ALT,SCr,DAO,ETX,IL-6,IL-8,IL-1β and TNF-α of the were reduced in Qingyi Ⅱ group at 24 hours and 72 hours of intervention(P＜0.05).(2)Protein expression in colon and rectal tissues:compared with the blank control group,p-Akt/Akt,p65,p-p65 were up-regulated in colon and rectal tissues of the model group,but the p-Akt/Akt,p65,p-p65 in the colon and rectum tissues of the Qingyi Ⅱ group was lower than that of the model group(P＜0.05).Conclusion Qingyi Ⅱ preparation can reduce the levels of serum cTnT,ALT,SCr,DAO,and has good repair and protective effects on multiple organs such as heart,liver,kidney and intestines in rats with wasp induced MODS by mediating the Akt/NF-κB signaling pathway.
Multiple organ dysfunction syndromeQingyi Ⅱ preparationWasp venomAction mechanismInflammatory factor
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