Objective To explore the comparison of trace blood and constant serum samples in the detection of vitamin D levels in chil-dren aged 0-6 years.Methods A prospective study was conducted,and 147 healthy children aged 0-6 years who visited the outpatient of De-partment of Pediatric Care,Yinchuan Maternal and Child Health Hospital from February 2020 to February 2022 were selected as the subjects.Mic-rosamples(peripheral blood sampling)and conventional blood samples(venous blood sampling)were collected from the enrolled subjects,and their levels of 25-hydroxyvitamin D3[25(OH)D3]were measured,with correction applied to the peripheral sampling results.The levels of 25(OH)D3 were compared between the two blood collection methods,and differences in 25(OH)D3 levels during different blood collection seasons(spring,summer,autumn,and winter)were also compared.The average puncture time,number of punctures,increase in respiratory rate,and pain level were recorded for both blood collection methods,and the satisfaction with the two blood collection methods was compared.Results The medion levels of 25(OH)D3 measured through venous and peripheral blood sampling were 29.10(24.40,34.30)ng/mol and 28.80(22.20,36.30)ng/mol,respectively,there was no statistically significant difference between the two methods(P＞0.05).There was statistically signif-icant difference in the detection results of 25(OH)D3 between venous and peripheral blood sampling taken in different blood collection seasons be-tween groups(P＜0.05),with the highest levels in summer[venous:29.78(22.46,36.48)ng/mol,peripheral:28.46(21.59,36.66)ng/mol]and the lowest in spring[venous:26.11(23.15,32.14)ng/mol,peripheral:25.41(22.56,33.46)ng/mol].There were no statistically significant differences in the average puncture time and visual analogue scale(VAS)scores between venous and peripheral blood collection(P＞0.05),however,the increase in respiratory rate was higher of peripheral blood sampling was(8.13±0.98％),which was higher than that of ve-nous blood sampling[(7.16±0.67％)],and the average number of punctures of peripheral blood sampling was(1.45±0.49)times,which was lower than that of venous blood sampling[(2.09±0.57)times],and the differences were statistically significant(P＜0.05).Additionally,the satisfaction rate of peripheral blood sampling was 95.24％,which was higher than that of venous blood sampling(87.07％),and the differ-ence was statistically significant(P＜0.05).Conclusion Vitamin D test was performed in children aged 0-6 years,and the results were not significantly different from constant blood test,but it could reduce the number of punctures and improve satisfaction,with the highest value in sum-mer and the lowest in spring.
维普
万方数据