Objective To investigate the effects of protein 0-fucosyltransferase 1(POFUT1)on the cell proliferation,migration,cell cycle,and apoptosis of esophageal squamous cell carcinoma(ESCC)cells and its potential mechanisms.Methods Human esophageal squamous carcinoma cells KYSE30 and EC 109 were transfected with siRNA and divided into POFUT1 knockdown group(transfected with POFUT1 siRNA)and negative control group(transfected with NC siRNA).The effect of knockdown was confirmed using quantitative reverse transcription polymer-ase chain reaction(qRT-PCR)and Western blotting.CCK-8 and Transwell assays were utilized to assess the impact of different POFUT1 ex-pression levels on cell proliferation and migration capabilities,and flow cytometry was employed to detect changes in cell cycle and apoptosis follow-ing POFUT1 knockdown.Genes that differed in expression between KYSE30 cells in the POFUT1 knockdown group and the negative control group were examined using transcriptome sequencing,and the KEGG database was utilized to analyze potential signaling pathways.Results Compared to the negative control group,both the quantity of migrating cells and the capacity for cell proliferation in the POFUT1 knockdown group were sig-nificantly decreased,the differences were statistically significant(P＜0.05).Furthermore,compared to the negative control group,the POFUT1 knockdown group had a larger percentage of apoptotic cells as well as a higher percentage of cells in the G2/M phase of the cell cycle,the differ-ences were statistically significant(P＜0.05).The results of transcriptome sequencing and qRT-PCR analyses showed that the downregulation of POFUT1 led to altered expression of several molecules involved in cancer-related signaling pathways,among which,the expression of genes S1PR5 and MAPK8 related to sphingolipid signaling pathway as well as those involved in the NOTCH signaling pathway,NOTCH1,NOTCH2,and DLL1,were significantly reduced.Conclusion Knockdown of POFUT1 in vitro leads to reduced proliferation and migration,cell cycle arrest,and increased apoptosis in esophageal squamous cell carcinoma cells.POFUT1 may affect the biological behavior of esophageal squamous cell car-cinoma cells through the sphingolipid signaling pathway and the NOTCH signaling pathway.
维普
万方数据