Objective To explore the effect of human nail bed-derived decellularized scaffolds serve as vector for bone marrow-derived mesenchymal stem cell differentiation into nail stem cells.Methods Clinically discarded nail bed tissue from 15 patients undergoing finger ampu-tation from 2022 to 2023 at Affiliated Hospital of Chengde Medical College was collected and prepared as decellularized nail bed scaffolds.Total collagen content and residual DNA content in the reference group(undecellularized group)versus decellularized group(decellularized nail bed scaffold group)were measured using the kit.Decellularized nail bed scaffolds were co-cultured with human bone marrow mesenchymal stem cells(hMSCs)for 14 d.The in vitro cell differentiation capacity and nail stem cell markers[keratin 15,keratin 17,leucine-rich repeat-containing G protein-coupled receptor 6(Lgr6),β-catenin protein]were compared between control group(hMSCs group)and co-cultured group(de-cellularized nail bed scaffolds+hMSCs group).Results The total collagen content in the decellularized group was(189.62±45.45)μg/mg,which was lower than that in the reference group[(196.02±41.93)μg/mg],but there was no statistically significant difference between the two groups(P＞0.05);the DNA content in the decellularized group was(0.41±0.15)μg/mg,which was significantly lower than that in the refer-ence group[(0.87±0.13)μg/mg],the difference was statistically significant(P＜0.05).After 14 days of cultivation,the absorbance value of the co culture group was 1.09±0.07,while the absorbance value of the control group was 1.10±0.50,there was no statistically significant difference between the two groups(P＞0.05).After 14 days of cultivation,the contents of keratin 15,keratin 17,Lgi6,β-catenin in the co-culture group were(39.56±5.09),(45.83±4.01),(5.74±0.99),and(146.79±5.34)μg/mL,which were significantly higher than those in the control group[(12.10±4.28),(10.47±3.19),(0.93±0.67),and(67.28±7.41)μg/mL],and the differences were statisti-cally significant(P＜0.05).Conclusion The decellularized nail bed scaffolds have cleared DNA and preserved collagen,and exhibit normal cell proliferation ability after co-culture with hMSCs and induce the differentiation of hMSCs to nail stem cells,which provide a new idea for the treatment of nail bed defects in the clinic.
关键词
角蛋白15/角蛋白17/人甲床/脱细胞支架/骨髓间充质干细胞/指甲干细胞
Key words
Keratin-15/Keratin-17/Human nail bed/Decellularized scaffold/Bone marrow-derived mesenchymal stem cells/Nail stem cell
维普
万方数据