Objective To study the value of real-time quantitative fluorescent polymerase chain reaction(qRT-PCR)combined with glass beads method in the detection of different tuberculosis specimens.Methods By retrospective analysis,qRT-PCR results of 1 200 different clinical specimens of suspected tuberculosis patients treated in inpatients and outpatients of Renji Hospital Affiliated to Shanghai Jiao Tong Univer-sity School of Medicine from January 2021 to June 2022 were collected and analyzed,including 469 blood samples,322 pleural effusion samples,16 pericardial effusion samples,and 25 urine samples.Trachea(bronchial)alveolar lavage fluid samples 8,abdominal fluid samples 34,cerebro-spinal fluid samples 143,pus samples 31,sputum samples 152;At the same time,41 sputum samples clinically diagnosed as pulmonary tubercu-losis were selected by establishing the pseudo-random function method,and the quantitative detection effect of qRT-PCR before and after glass bead oscillating grinding bacteria was analyzed.Results The total positive rate of 1 200 tuberculosis specimens was 15.83％(190/1 200),a-mong which the highest positive rate was 37.50％(3/8)in trachea(bronchial)alveolar lavage fluid specimen,and the highest positive rate was 40.00％(2/5)in outpatients.The positive rate of pus specimen was the second highest in 35.48％(11/31),and the highest in inpatients was 38.46％(10/26).The detection rate of sputum specimen was the third,the proportion was 30.26％(46/152).The concentration of nucleic acid detected by qRT-PCR was significantly higher than that before treatment,the difference was statistically significant(P＜0.05).Among them,14 sputum samples were increased by the order of strain,and 11 sputum samples with 102 copies/mL bacteria content were increased to the order of 103 copies/mL bacteria content after the oscillatory grinding treatment,and 7 sputum samples were increased to the order of 103 copies/mL bacteria content.The positive detection rate after the grinding treatment was significantly higher than that before the grinding treatment,the difference was statistically significant(P＜0.05).Conclusion The detection rate of qRT-PCR is higher in trachea(bronchial)alveolar lav-age fluid,pus and sputum specimens,while the detection rate of qRT-PCR is lower in other tuberculosis specimens.qRT-PCR combined with vitreous beads can effectively improve the positive detection rate of clinical tuberculosis specimens.
维普
万方数据