摘要
目的 探讨2型糖尿病(T2DM)患者血清中miRNA-27、纤维细胞生长因子21(FGF21)及糖化终末产物(AGE)的表达,并分析它们与微血管并发症的相关性.方法 回顾性选取2020年2月至2022年2月在沧州市人民医院进行诊疗的89例2型糖尿病患者纳入研究组,根据患者是否合并微血管并发症(包括糖尿病所致的周围神经病变、肾脏病变和视网膜病变)将其分为合并组(n=41)和未合并组(n=48).另选50例在本院进行健康体检的志愿者纳入对照组.比较研究组和对照组的miRNA-27、FGF21及AGE水平;比较合并组和未合并组患者的miRNA-27、FGF21、AGE水平、生化指标[总胆固醇、甘油三酯、甘油三酯、高密度脂蛋白(HDL-C)、低密度脂蛋白胆固醇(LDL-C)、尿酸、血肌酐及尿素氮]水平,并探究影响患者合并微血管并发症的影响因素.结果 研究组的miRNA-27、FGF21、AGE 水平分别为 2.31±0.42、(388.27±23.09)ng/mL、(89.29±4.98)UA,均高于对照组[0.89±0.31、(268.56±26.18)ng/mL、(70.22±5.16)UA],差异均有统计学意义(P＜0.05).合并组患者的miRNA-27、FGF21、AGE水平分别为2.45±0.44、(399.26±25.49)ng/mL、(92.34±4.28)UA,均高于未合并组[1.98±0.36、(316.44±23.91)ng/mL、(76.28±3.92)UA],差异均有统计学意义(P＜0.05).两组间总胆固醇、LDL-C水平比较,差异均无统计学意义(P＞0.05);合并组患者的HDL-C水平为(1.12±0.33)mmo/L,低于未合并组[(1.28±0.36)mmo/L],甘油三酯、尿酸、血肌酐、尿素氮水平分别为(2.33±0.51)mmo/L、(355.26±41.08)μmol/L、(92.08±9.04)μmol/L、(7.99±2.15)mmol/L,均高于未合并组[(1.98±0.46)mmo/L、(336.11±45.26)μmol/L、(72.45±9.87)μmol/L、(5.06±1.03)mmol/L],差异均有统计学意义(P＜0.05).多因素Logistic回归分析,可知FGF21、AGE、miRNA-27均为2型糖尿病合并微血管并发症产生的影响因素(P＜0.05).结论 2型糖尿病患者的血清中miRNA-27、FGF21和AGE水平较高,且在合并微血管并发症的2型糖尿病患者中表达更高,与2型糖尿病的微血管并发症的发生具有一定的相关性.
Abstract
Objective To investigate the expression of miRNA-27,fibrocyte growth factor 21(FGF21)and advanced glycation end product(AGE)in serum of patients with type 2 diabetes mellitus(T2DM),and to analyze their correlation with vascular complications.Methods A total of 89 patients with T2DM who received treatment in Cangzhou People's Hospital from February 2020 to February 2022 were retrospectively included in the study group,and divided into the combined group(n=41)and the non combined group(n=48)according to whether they had microvascular complications(including peripheral neuropathy,nephropathy and retinopathy caused by diabetes).And 50 volunteers who under-went physical examination in our hospital were included in the control group.The serum miRNA-27,FGF21,AGE of the study group and the control group were compared.The serum miRNA-27,FGF21,AGE and biochemical indicators[total cholesterol,triglycerides,triglycerides,high-density lipoprotein(HDL-C),low-density lipoprotein cholesterol(LDL-C),uric acid,blood creatinine,and urea nitrogen]of the two groups were compared,the relationship between the above indexes and microvascular complications was analyzed,and the influencing factors affecting microvascular complications was explored.Results The levels of miRNA-27,FGF21,and AGE in the study group were 2.31±0.42,(388.27±23.09)ng/mL,and(89.29±4.98)UA,respectively,which were higher than those in the control group[0.89±0.31,(268.56± 26.18)ng/mL,and(70.22±5.16)UA],and the differences were statistically significant(P＜0.05).The levels of miRNA-27,FGF21,and AGE in the combined group were 2.45±0.44,(399.26±25.49)ng/mL,and(92.34±4.28)UA,respectively,which were higher than those in the non combined group[1.98±0.36,(316.44±23.91)ng/mL,and(76.28±3.92)UA],and the differences were statistically significant(P＜0.05).There were no statistically significant differences in total cholesterol and LDL-C levels between the two groups(P＞0.05);the level of HDL-C in the combined group was(1.12±0.33)mmo/L,which was lower than that in the non combined group[(1.28 ±0.36)mmo/L],the levels of triglycerides,uric acid,blood creatinine,and urea nitrogen were(2.33±0.51)mmo/L and(355.26±41.08)μmol/L,(92.08±9.04)μmol/L and(7.99±2.15)mmol/L,respectively,which were higher than those in the unconjugated group[(1.98± 0.46)mmo/L and(336.11±45.26)]μmol/L,(72.45±9.87)μmol/L,(5.06±1.03)mmol/L,the differences were statistically signifi-cant(P＜0.05).Multivariate logistic regression analysis showed that FGF21,AGE and miRNA-27 were the influencing factors of T2DM with microvascular complications(P＜0.05).Conclusion The serum levels of miRNA-27,FGF21 and AGE are higher in patients with T2DM,and the expression is higher in patients with T2DM complicated with vascular complications,which has a certain correlation with the occurrence of vascular complications of T2DM.
NETL
NSTL
维普
万方数据