摘要
目的 研究组织内缺氧诱导因子-1α(HIF-1α)、骨桥蛋白表达与结直肠癌患者新辅助化疗疗效的关系.方法 回顾性分析2020年6月至2022年6月秦皇岛市第一医院收治的140例结直肠癌患者的临床资料.所有患者均行3个疗程XELOX新辅助化疗,依据3个疗程后的化疗疗效将患者分为有效组(n=88)和无效组(n=52).比较HIF-1α和骨桥蛋白表达与结直肠癌患者临床病理特征的关系,比较两组基础资料信息(性别、年龄、淋巴结转移、分化程度、TNM分期、肿瘤大小、HIF-1α表达及骨桥蛋白表达)差异,采用多因素Logistic回归分析影响结直肠癌新辅助化疗无效的危险因素,采用Pearson相关性分析组织内HIF-1α和骨桥蛋白阳性表达与结直肠癌新辅助化疗疗效的关系.结果 组织内HIF-1α、骨桥蛋白表达与患者淋巴结转移有关(P＜0.05).两组性别构成比、年龄、淋巴结转移、分化程度、TNM分期比较,差异均无统计学意义(P＞0.05);无效组的肿瘤≥3 cm、HIF-1α阳性表达以及骨桥蛋白阳性表达患者比率分别为65.38％、88.46％、90.38％,均显著高于有效组(45.45％、70.45％、63.64％),差异均有统计学意义(P＜0.05).经多因素Logistic回归分析证实,肿瘤≥3 cm、HIF-1α阳性及骨桥蛋白阳性均是影响结直肠癌新辅助化疗无效的危险因素(P＜0.05).相关性分析显示,组织内HIF-1α及骨桥蛋白阳性表达与结直肠癌新辅助化疗疗效呈负相关(r=-0.553、-0.486,P＜0.05).结论 组织内HIF-1α及骨桥蛋白阳性表达与结直肠癌新辅助化疗疗效呈负相关,且肿瘤≥3 cm、HIF-1α阳性及骨桥蛋白阳性均是影响结直肠癌新辅助化疗无效的危险因素,可将以上指标作为评估结直肠癌个体化新辅助化疗疗效的生物学标志物,为临床病情评估和治疗提供参考.
Abstract
Objective To investigate the relationship between the expression of hypoxia inducible factor 1α(HIF-1α)and osteopontin in tissues and the efficacy of neoadjuvant chemotherapy in patients with colorectal cancer.Methods The clinical data of 140 colorectal cancer pa-tients admitted to the First Hospital of Qinhuangdao from June 2020 to June 2022 were retrospectively analyzed.All patients received three courses of XELOX neoadjuvant chemotherapy,and the patients were divided into the effective group(n=88)and the ineffective group(n=52)accord-ing to the chemotherapy efficacy after three courses.The relationship between HIF-1 α and osteopontin expression and clinicopathological features of colorectal cancer patients was compared,and the differences of basic data(gender,age,lymph node metastasis,differentiation,TNM stage,tumor size,HIF-1α expression and osteopontin expression)between the two groups were compared.The risk factors affecting the ineffectiveness of neoadjuvant chemotherapy for colorectal cancer were analyzed by multivariate Logistic regression.The relationship between HIF-1α and os-teopontin expression and the efficacy of neoadjuvant chemotherapy in colorectal cancer was analyzed by Pearson correlation.Results HIF-1αand osteopontin expression were associated with lymph node metastasis(P＜0.05).There were no statistically significant differences in gender composition ratio,age,lymph node metastasis,degree of differentiation,and TNM staging between the two groups(P＞0.05);The proportions of patients with tumor ≥3 cm,HIF-1α positive expression and osteopontin positive expression in the ineffective group were 65.38％,88.46％,and 90.38％,respectively,which were significantly higher than those of the effective group(45.45％,70.45％,63.64％),and the differences were statistically significant(P＜0.05).Multivariate Logistic regression analysis confirmed that tumor ≥3 cm,HIF-1α positive and osteopon-tin positive were risk factors affecting the effectiveness of neoadjuvant chemotherapy for colorectal cancer(P＜0.05).Correlation analysis showed that the positive expression of HIF-1 α and osteopontin in tissues was negatively correlated with the efficacy of neoadjuvant chemotherapy for color-ectal cancer(r=-0.553,-0.486;P＜0.05).Conclusion Positive expression of HIF-1α and osteopontin in tissues is negatively correla-ted with the efficacy of neoadjuvant chemotherapy in colorectal cancer,and tumor ≥3 cm,HIF-1α positive and osteopontin positive are all risk factors affecting the efficacy of neoadjuvant chemotherapy in colorectal cancer.These indicators can be used as biological markers to evaluate the efficacy of individualized neoadjuvant chemotherapy in colorectal cancer,and to provide reference for clinical assessment and treatment.
基金项目
河北省卫生健康委医学科学计划项目(201902A137)
NETL
NSTL
维普
万方数据