摘要
目的 探讨胃癌模型大鼠胃黏膜组织细胞周期蛋白D1(cyclinD1)、c-Myc、CKIT的表达意义及其与胃癌病变程度的相关性.方法 选择48只健康成年雌性大鼠,按照随机数字表法将其分为对照组、研究1组、研究2组、研究3组,每组各12只.研究1组、研究2组、研究3组均制备成胃癌模型.对照组给予等量0.9％氯化钠溶液,第24周处死取材;研究1组、研究2组、研究3组制备成胃癌大鼠后分别于第8、16、24周取材.分析各组大鼠一般情况及胃黏膜组织病理切片,采用蛋白质印迹法检测胃黏膜组织Cyclin D1、c-Myc、CKIT蛋白的表达,采用逆转录聚合酶链式反应(RT-PCR)法测定胃黏膜组织Cyclin D1、c-Myc、CKIT mRNA的表达,采用Spearman分析法测定胃黏膜组织Cyclin D1、c-Myc、CKIT表达与胃癌病变程度相关性.结果 对照组大鼠胃黏膜完整正常,外膜层、肌层、黏膜下层、黏膜层等结构清晰,且无炎症细胞浸润;研究1组大鼠胃黏膜组织与对照组大鼠接近,不存在炎症细胞,且黏膜腺体结构基本正常;研究2组大鼠胃黏膜组织存在破损,细胞核变大,基底部部分腺体细胞形态异常,存在轻度异型性,为早期胃癌;研究3组大鼠胃黏膜组织增加破损,核质比变大,细胞形态不规则,部分腺体存在扩张,黏膜下层及肌层存在炎症细胞浸润,为胃癌进展期.研究1组、研究2组、研究3组胃黏膜组织Cyclin D1、c-Myc、CKIT蛋白均呈显著高于对照组,差异均有统计学意义(P＜0.05),胃黏膜组织Cyclin D1、c-Myc、CKIT蛋白在研究1组、研究2组、研究3组中逐渐升高趋势.研究1组、研究2组、研究3组胃黏膜组织Cyclin D1、c-Myc、CKIT mRNA均呈显著高于对照组,差异均有统计学意义(P＜0.05),胃黏膜组织Cyclin D1、c-Myc、CKIT mRNA在研究1组、研究2组、研究3组中逐渐升高趋势.采用Spearman相关性结果分析显示,胃黏膜组织Cyclin D1、c-Myc、CKIT表达与胃癌病变程度呈正相关(r=0.382、0.781、0.993,均P＜0.001).结论 胃癌模型大鼠胃黏膜组织Cyclin D1、c-Myc、CKIT呈高表达,其与胃癌病变程度密切相关.
Abstract
Objective To investigate the expression significance of Cyclin D1,c-Myc and CKIT in gastric mucosal tissues of gastric cancer model rats and their correlation with the degree of gastric cancer lesions.Methods Forty-eight healthy adult female rats were selected and divided into the control group,the study 1 group,study 2 group,and study 3 group according to the random number table method,with 12 rats in each group.The study 1 group,study 2 group,and study 3 group all established gastric cancer models.The control group was given an e-qual amount of 0.9％sodium chloride solution,and samples were euthanized at 24 weeks.The study 1 group,study 2 group,and study 3 group were prepared into gastric cancer rats and collected samples at 8,16,and 24 weeks,respectively.The general conditions and pathological sections of gastric mucosa of rats in each group were analyzed.The expressions of Cyclin D1,c-Myc and CKIT proteins in gastric mucosa were detected by Western blotting,and the expressions of Cyclin D1,c-Myc and CKIT mRNA in gastric mucosa were detected by reverse transcription polymer-ase chain reaction(RT-PCR).Results The gastric mucosa of the control group rats was intact and normal,with clear structures such as the outer layer,muscular layer,submucosal layer,and mucosal layer,and no inflammatory cell infiltration;the gastric mucosal tissue of the study 1 group rats was similar to that of the control group rats,with no inflammatory cells present and the mucosal glandular structure was basically normal;the gastric mucosa of the study 2 group rats was damaged,with enlarged cell nuclei,abnormal morphology of glandular cells in the basal part,and mild dysplasia,indicating early gastric cancer;the gastric mucosal tissue of the study 3 group rats showed increased damage,increased nuclear to cytoplasmic ratio,irregular cell morphology,expansion of some glands,and inflammatory cell infiltration in the submucosal and muscular layers,indicating the progression of gastric cancer.The levels of Cyclin D1,c-Myc,and CKIT proteins in gastric mucosal tissue of the study 1 group,study 2 group,and study 3 group were significantly higher than those in the control group,the differences were statistically significant(P＜0.05);the levels of Cyclin D1,c-Myc,and CKIT proteins in gastric mucosal tissue gradually increased in the study 1 group,study 2 group,and study 3 group.The levels of Cyclin D1,c-Myc,and CKIT mRNA in gastric mucosal tissue of the study 1 group,study 2 group,and study 3 group were significantly higher than those in the control group,the differences were statistically significant(P＜0.05);the levels of Cyclin D1,c-Myc,and CKIT mRNA in gastric mucosal tissue gradually increased in the study 1 group,study 2 group,and study 3 group.Spearman correlation analy-sis showed that the expression of Cyclin D1,c-Myc,CKIT in gastric mucosa was positively correlated with the degree of gastric cancer lesions(r=0.382,0.781,0.993,P＜0.001).Conclusion Cyclin D1,c-Myc and CKIT are highly expressed in gastric mucosa of gastric cancer model rats,which are closely related to the degree of gastric cancer lesions.
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维普
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