摘要
目的 探讨获得性免疫缺陷综合征(AIDS)合并脓毒症患者外周血中T淋巴细胞亚群.方法 回顾性选取2021年10月至2023年9月海南医学院第二人民医院内AIDS合并脓毒症患者60例作为试验组,AIDS无脓毒症患者60例作为对照1组,健康献血员20名作为对照2组.应用免疫荧光和单克隆抗体标记技术检测试验组、对照1组和对照2组外周血T淋巴细胞亚群(CD3细胞数量、CD4细胞数量、CD8细胞数量、CD4/CD8比值),比较试验组、对照1组和对照2组T淋巴细胞亚群计数和比例,并统计分析试验组不同年龄、确诊时间患者的T淋巴细胞亚群计数和比例.结果 试验组患者的CD3细胞数量、CD4细胞数量分别为(864.72±184.22)、(145.37±25.37)个/μL,均明显少于对照1 组[(971.80±135.14)、(218.11±36.24)个/μL]、对照 2 组[(1 098.12±156.23)、(296.23±41.25)个/μL],CD4/CD8比值为0.258±0.024,明显低于对照1组(0.344±0.034)、对照2组(0.458±0.045),差异均有统计学意义(P＜0.05);对照1组患者的CD3细胞数量、CD4细胞数量均明显少于对照2组,CD4/CD8比值明显低于对照2组,差异均有统计学意义(P＜0.05);但3组研究对象的CD8细胞数量比较,差异均无统计学意义(P＞0.05).不同年龄患者的CD3细胞数量、CD4细胞数量、CD8细胞数量、CD4/CD8比值比较,差异均无统计学意义(P＞0.05).不同确诊时间患者的CD4细胞数量比较,差异有统计学意义(P＜0.05),其中,确诊时间0～1年、1～2年患者的CD4细胞数量均多于2～3年、3～4年、4～5年、≥5年患者(P＜0.05);但不同确诊时间患者的CD3细胞数量、CD8细胞数量、CD4/CD8比值比较,差异均无统计学意义(P＞0.05).结论 AIDS合并脓毒症患者外周血中T淋巴细胞亚群降低,CD4细胞数量与确诊时间密切相关,不仅能拓宽临床对于AIDS机会性感染认识,更能为其诊治提供新的思路及方向.
Abstract
Objective To investigate the T lymphocyte subsets in peripheral blood of acquired immune deficiency syndrome(AIDS)pa-tients with sepsis.Methods From October 2021 to September 2023,60 cases of HIV/AIDS patients with sepsis in the Second Affiliated Hospital of Hainan Medical College were selected as the experimental group,60 cases of AIDS patients without sepsis were selected as the control group 1,and 20 cases of healthy blood donors were selected as the control group 2.T lymphocyte subsets(CD3 cells,CD4 cells,CD8 cells,CD4/CD8 rati-o)in peripheral blood of the experimental group,the control group 1 and the control group 2 were detected by immunofluorescence and monoclonal antibody labeling techniques,and the count and proportion of T lymphocyte subsets of test group,control group 1 and control group 2 were com-pared.And the T lymphocyte subsets count and proportion of patients of different ages and diagnosis times in the experimental group were statisti-cally analyzed.Results The number of CD3 cells and CD4 cells in the experimental group patients were(864.72±184.22)and(145.37± 25.37)/μL,respectively,which were significantly less than those in the control group 1[(971.80±135.14),(218.11±36.24)]/μL]and the control group 2[(1 098.12±156.23),(296.23±41.25)/μL],the CD4/CD8 ratio was 0.258±0.024,which was significantly lower than that in the control group 1(0.344±0.034)and the control group 2(0.458±0.045),the differences were statistically significant(P＜0.05);the number of CD3 cells and CD4 cells in the control group 1 were significantly lower than those in the control group 2,and the CD4/CD8 ratio was significantly lower than that in the control group 2,the differences were statistically significant(P＜0.05);however,there was no sta-tistically significant difference in the number of CD8 cells among the three study groups(P＞0.05).There was no statistically significant differ-ence in the number of CD3 cells,CD4 cells,CD8 cells,and CD4/CD8 ratio among patients of different ages(P＞0.05).There was statistically significant difference in the number of CD4 cells among patients diagnosed at different times(P＜0.05).The number of CD4 cells in patients with 0-1 year and 1-2 years after diagnosis was higher than that in patients with 2-3 years,3-4 years,4-5 years and ≥ 5 years,the differ-ences were statistically significant(P＜0.05);However,there was no statistically significant difference in the number of CD3 cells,CD8 cells,and CD4/CD8 ratio among patients diagnosed at different times(P＞0.05).Conclusion T lymphocyte subsets in peripheral blood of AIDS pa-tients with sepsis decreased,CD4 cells are closely related to diagnosis time,which can not only broaden our understanding of AIDS opportunistic infection,but also provide new ideas and directions for its diagnosis and treatment.
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维普
万方数据