摘要
目的 对慢性牙周炎患者龈沟液人β防御素-3(hBD-3)水平与牙周指标及炎症因子之间的关系进行探究.方法 选取2020年5月至2022年5月于成都医学院第二附属医院·核工业四一六医院收治的慢性牙周炎患者120例作为研究对象,根据病情程度分为中重度组(n=52)和轻度组(n=68),选取同期70名健康人员为对照组,比较3组龈沟液hBD-3、牙周指标[牙龈菌斑指数(PLI)、牙龈指数(GI)、探诊出血指数(BOP)、牙周袋深度(PD)、附着丧失(AL)]及血清炎症因子[高敏C-反应蛋白(hs-CRP)、白细胞计数(WBC)、肿瘤坏死因子-α(TNF-α)、白细胞介素(IL)-6、IL-1α、IL-1β]水平.Pearson法、多元线性回归分析龈沟液hBD-3水平与牙周指标及炎症因子的相关性.结果 对照组、轻度组和中重度组的龈沟液hBD-3水平分别为(4.69±1.45)、(2.85±0.62)、(1.32±0.28)μg/L,差异有统计学意义(P＜0.05),对照组、轻度组和中重度组的龈沟液hBD-3水平逐渐降低.3组牙周指标PLI、GI、BOP、PD水平比较,差异均有统计学意义(P＜0.05),对照组、轻度组和中重度组的PLI、GI、BOP、PD水平逐渐升高,AL水平逐渐降低.3组炎症因子水平比较,差异均有统计学意义(P＜0.05),对照组、轻度组和中重度组的hs-CRP、TNF-α、IL-6、IL-1α、IL-1β、WBC 水平逐渐升高.Pearson 相关性显示,hBD-3 水平分别与 PD、hs-CRP、TNF-α、IL-6 呈负相关(r=-0.500、-0.682、-0.508、-0.560,P＜0.05),与 AL 呈正相关(r=0.589,P＜0.05).多元线性回归分析显示,PD、AL、hs-CRP、TNF-α、IL-6与龈沟液hBD-3水平有密切的关联影响关系(P＜0.05).结论 慢性牙周炎患者的龈沟液hBD-3水平下降,并且hBD-3水平分别与牙周指标及炎症因子密切相关.
Abstract
Objective To explore the correlation between the level of human beta-defensin-3(hBD-3)in gingival crevicular fluid(GCF)and periodontal indicators and inflammatory factors in patients with chronic periodontitis.Methods A total of 120 patients with chronic periodontitis who were diagnosed and treated in 416 Nuclear Industry Hospital,Second Affiliated Hospital of Chengdu Medical College from May 2020 to May 2022 were regarded as the study subjects,according to the severity of the condition,they were divided to the mild group(n=68)and the moderate to severe group(n=52),at the same time,70 healthy individuals who had no oral diseases examined in the same hospital were collected as the control group,the level of hBD-3 in gingival crevicular fluid,periodontal indicators[plaque index(PLI),gingival index(GI),bleeding on probing(BOP),pocket depth(PD),apparatus loss(AL)]and serum inflammatory factors[high-sensitivity C-reactive protein(hs-CRP),white blood cell count(WBC),tumor necrosis factor alpha(TNF-α),interleukin(IL)-6,IL-1α,IL-1β]were compared a-mong the three groups.Pearson's method and multiple linear regression analysis were applied to investigate the correlation between gingival sulcus fluid hBD-3 levels and periodontal indicators and inflammatory factors.Results The levels of gingival crevicular fluid hBD-3 in the control group,the mild group,and the moderate to severe group were(4.69±1.45),(2.85±0.62),and(1.32±0.28)pg/L,respectively,the differences were statistically significant(P＜0.05);the levels of gingival crevicular fluid hBD-3 in the control group,the mild group,and the moderate to severe group gradually decreased.There were statistically significant differences in the levels of periodontal indicators PLI,GI,BOP,and PD in the three groups(P＜0.05),the levels of PLI,GI,BOP,and PD in the control group,the mild group,and the moderate to severe group gradually increased,while the levels of AL gradually decreased.There were statistically significant differences in the levels of inflammatory factors in the three groups(P＜0.05),the levels of hs-CRP,TNF-α,IL-6,IL-1α,IL-1 β,and WBC in the control group,the mild group,and the moderate to severe group gradually increased.hBD-3 levels were negatively correlated with PD,hs-CRP,TNF-α,and IL-6(r=-0.500,-0.682,-0.508,-0.560,P＜0.05),respectively,and positively correlated with AL(r=0.589,P＜0.05).Multiple linear regression analysis showed that there was a close correlation between PD,AL,hs-CRP,TNF-α,IL-6 and the level of hBD-3 in gingi-val crevicular fluid(P＜0.05).Conclusion Gingival sulcus fluid hBD-3 levels are decreased in patients with chronic periodontitis,and hBD-3 levels are strongly correlated with periodontal indicators and inflammatory factors,respectively.
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