Objective To study the effect of moxibustion on the expression of serum tumor necrosis factor-α(TNF-α),interlukin-6(IL-6),C-C motif chemokine ligand 22(CCL22)in guinea pigs.Methods Five of the 25 guinea pigs were taken as the blank control group,and the rest were taken as the treatment group.According to the random number table method,the remaining 20 guinea pigs were divided into 4 groups according to different time points,which were immediately after moxibustion treatment group(T-1 group),1 month after moxibus-tion treatment group(T-2 group),2 months after moxibustion treatment group(T-3 group),and 3 months after moxibustion treatment group(T-4 group),with 5 guinea pigs in each group.The back depilation of guinea pigs in each group was about 2 cm × 2 cm.Except for the blank control group,the other four groups of guinea pigs were treated with moxibustion.The moxibustion treatment points were"Jiudianfeng"and"Ashi"."Ashi acupoint"is the depilation area.Hanging moxibustion at 3cm above acupoints adopts mild moxibustion and tonifying method,each acupoint is about 10min once a day,and the course of treatment is 1 month.The blank control group was only fixed by the same method without moxibustion.The expression levels of serum TNF-α,IL-6 and CCL22 in the five groups were compared.Results The expression levels of ser-um TNF-α in T-1 group,T-2 group and T-4 group were(42.49±4.66),(88.75±23.18)and(79.76±20.20)ng/L,respectively,which were significantly lower than those in blank control group[(119.55±11.25)ng/L],and the differences were statistically significant(P＜0.05).There was no significant difference in the expression level of serum TNF-α between the T-3 group and the blank control group(P＞0.05).The expression level of serum IL-6 in the T-1 group was(37.70±8.94)ng/L,which was significantly lower than that in the blank control group[(51.51±9.95)ng/L],and the difference was statistically significant(P＜0.05).There was no significant difference in serum IL-6 expression level between T-2 group,T-3 group,T-4 group and blank control group(P＞0.05).The expression levels of CCL22 in serum of guinea pigs in T-1 group,T-2 group,T-3 group and T-4 group were(176.14±27.62),(197.06±34.05),(146.22±9.85)and(216.52±38.65)ng/L,respectively,which were significantly higher than those in the blank control group[(120.86±23.89)ng/L],and the differences were statistically significant(P＜0.05).Conclusion Moxibustion can decrease the level of serum TNF-α、IL-6 in guinea pigs whereas increasethe level of serum CCL22 in guinea pigs,and the effect of moxibustion on TNF-α CCL22 can last 3 months after the end of moxibustion.
维普
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