摘要
目的 探讨血清胶质纤维酸性蛋白(GFAP)、甲基化CpG结合蛋白2(MeCP2)对脑胶质瘤的诊断价值及其与病理特征的相关性.方法 回顾性选取2021年1月至2024年1月南京医科大学附属脑科医院(南京脑科医院)收治的80例脑胶质瘤患者,将其作为胶质瘤组,选取本院同期收治的80例非胶质瘤颅内良性肿瘤患者作为对照组.应用双抗体夹心法检测两组患者血清GFAP表达水平,应用荧光定量聚合酶链反应法检测肿瘤组织MeCP2表达水平.比较脑胶质瘤患者与对照组的GFAP、MeCP2表达水平,并建立ROC曲线分析GFAP、MeCP2对脑胶质瘤的诊断价值.分析不同病理特征脑胶质瘤患者GFAP、MeCP2表达水平,并采用Spearman相关性分析GFAP、MeCP2与脑胶质瘤病理特征的相关性.结果 胶质瘤组患者GFAP、MeCP2表达水平分别为(81.74±7.47)ng/L、2.31±0.31,均高于对照组[(5.35±1.32)ng/L、0.72±0.16],差异均有统计学意义(P＜0.05).绘制ROC曲线结果显示,GFAP、MeCP2、两者联合诊断脑胶质瘤的曲线下面积(AUC)分别为0.721、0.698、0.897.GFAP、MeCP2两者联合对脑胶质瘤的诊断AUC高于两者单一诊断.病理分级Ⅳ级、远处转移患者GFAP水平均高于其他分级、未远处转移,差异均有统计学意义(P＜0.05).病理分级Ⅳ级、肿瘤大小≥3 cm、远处转移患者MeCP2水平均高于其他分级、肿瘤大小＜3 cm、未远处转移患者,差异均有统计学意义(P＜0.05).Spearman相关分析结果表明,GFAP与脑胶质瘤不同病理分级、远处转移均呈正相关(r=0.579、0.378,P＜0.05);MeCP2与脑胶质瘤不同病理分级、远处转移、脑胶质瘤肿瘤大小均呈正相关(r=0.657、0.457、0.384,P＜0.05).结论 胶质纤维酸性蛋白、甲基化CpG结合蛋白2对于脑胶质瘤均具有重要诊断参考价值,两者联合诊断敏感度、特异度更高,且两者与脑胶质瘤的病理分级、肿瘤大小、远处转移情况密切相关.
Abstract
Objective To explore the diagnostic value of serum glial fibrillary acidic protein(GFAP)and methylated CpG binding pro-tein 2(MeCP2)in gliomas and their correlation with pathological features.Methods Eighty patients with gliomas admitted to The Affiliated Brain Hospital of Nanjing Medical University(Nanjing Brain Hospital)from January 2021 to January 2024 were selected as the glioma group,and 80 patients with non glioma intracranial benign tumors admitted to the same hospital during the same period were selected as the control group.The serum GFAP expression levels of the two groups of patients were detected using the double antibody sandwich method,and the MeCP2 expression level in tumor tissue was detected using fluorescence quantitative polymerase chain reaction.The expression levels of GFAP and MeCP2 in the glio-ma group and control group were compared.The diagnostic value of GFAP and MeCP2 for gliomas was analyzed by establishing receiver operating characteristic(ROC)curve.The expression levels of GFAP and MeCP2 in glioma patients with different pathological features were analyzed,and the correlation between GFAP,MeCP2 and pathological features of glioma was investigated by Spearman correlation analysis.Results The expres-sion levels of GFAP and MeCP2 in the glioma group were(81.74±7.47)ng/L and 2.31±0.31,respectively,which were higher than those in the control group[(5.35±1.32)ng/L,0.72±0.16],the differences were statistically significant(P＜0.05).The ROC curve results show that the area under the curve(AUC)for GFAP,MeCP2,and their combined diagnosis of glioma were 0.721,0.698,and 0.897,respectively.The levels of GFAP in patients with pathological grade Ⅳ and distant metastasis were higher than those in other grades and without distant metasta-sis,and the differences were statistically significant(P＜0.05).Patients with pathological grade Ⅳ,tumor size ≥3 cm,and distant metastasis had higher MeCP2 levels than those with other grades,tumor size＜3 cm,and no distant metastasis,and the differences were statistically signifi-cant(P＜0.05).The Spearman correlation analysis results showed that GFAP was positively correlated with different pathological grades,distant metastasis of glioma(r=0.579,0.378;P＜0.05);MeCP2 was positively correlated with different pathological grades,distant metastasis,and tumor size of glioma(r=0.657,0.457,0.384;P＜0.05).Conclusion Glial fibrillary acidic protein and methylated CpG binding protein 2 have important diagnostic reference value for gliomas.The combined diagnosis of the two has higher sensitivity and specificity,and they are closely related to the pathological grading,tumor size,and distant metastasis of gliomas.
基金项目
国家自然科学基金青年科学基金项目(82203637)
维普
万方数据