Objective To investigate the clinical value of bronchoalveolar lavage fluid(BALF)metagenomic next-generation sequencing(mNGS)in pathogen detection of patients with pulmonary infection.Methods The clinical data of 98 cases of pulmonary infection hospitalized in the Department of Respiratory and Critical Care Medicine of the Second Peoples Hospital of Hefei from April 2021 to December 2023 were retro-spectively studied.All patients underwent routine pathogen detection and BALF mNGS detection.The detection rate of pathogenic bacteria,the consistency of diagnosis results and the diagnostic efficacy of the two methods in BALF were compared.Results A total of 54 bacteria,14 fungi,13 viruses,3 mycoplasma and 3 chlamydia were detected in bronchoalveolar lavage fluid with mNGS in BALF.The total detection rate of bacteria,fungi,viruses and pathogens of mNGS in bronchoalveolar lavage fluid was 88.78％,which was significantly increased compared with conventional test(25.51％),the difference was statistically significant(P＜0.05).The Kappa between the detection results of pathogenic microorganisms mNGS in BALF and the confirmed results was 0.795,with a high consistency(P＜0.001).The sensitivity and diagnostic load rate of pathogenic microorganism mNGS in pulmonary infection patients was 95.92％,which was significantly higher than that of routine examination(28.57％),the difference was statistically significant(P＜0.05).Conclusion Compared with conventional pathogen detection methods,the application of mNGS detection in BALF significantly improves the detection rate of microorganisms such as bacteria,fungi,viruses,mycoplasma,and chlamydia,and can identify more types of microorganisms.MNGS technology has shown high sensitivity and diagnostic accuracy in diagnosing pul-monary infections.
维普
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