摘要
目的 探讨肺炎支原体肺炎(MPP)患儿血清白细胞介素(IL)-25、IL-33及嗜酸细胞趋化因子(Eotax-in)水平与肺功能的相关性.方法 回顾性选取2021年6月至2023年6月入大庆龙南医院的60例MPP患儿,设为观察组;同时选取同期入院体检的60名健康儿童设为对照组.采集两组儿童的血样,对血清学指标[半胱氨酰白三烯(CysLTs)、嗜酸性粒细胞阳离子蛋白(ECP)、Eotaxin、IL-33、IL-25、IL-6、IL-4、肿瘤坏死因子α(TNF-α)]予以测定,同时测定肺功能指标[潮气量、达峰时间比(tPTEF/TE)、呼出75％潮气量时的瞬间流速/潮气呼气峰流速比(TEF25/PTEF％)、用力肺活量(FVC)、最大深呼吸后第1秒呼出气量容积(FEV1)、最大呼气峰流速值(PEF)],对Eotaxin、IL-33、IL-25与肺功能相关性予以分析.结果 观察组患儿的血清CysLTs、ECP、Eotaxin、IL-33、IL-25、IL-6、IL-4、TNF-α 水平分别为(637.85±98.42)ng/L、(28.91±4.43)μg/L、(110.96±18.85)ng/L、(11.76±1.53)ng/L、(46.23±7.31)ng/L、(58.54±9.46)pg/mL、(42.81±7.53)ng/L、(3.02±0.89)mg/L,均明显高于对照组[(493.49±78.54)ng/L、(14.95±2.43)μg/L、(101.76±18.53)ng/L、(10.21±0.98)ng/L、(39.62±6.74)ng/L、(24.63±4.58)pg/mL、(26.52±4.49)ng/L、(0.92±0.28)mg/L],差异均有统计学意义(P＜0.05).观察组患儿的潮气量、tPTEF/TE、TEF25/PTEF％、FVC、FEV1、PEF 分别为(8.01±1.18)mL/kg、(36.16±6.54)％、(66.03±11.34)％、(66.57±10.02)％、(69.91±9.35)％、(73.14±9.83)％,均明显低于对照组[(9.19±1.28)mL/kg、(46.03±6.92)％、(78.63±14.75)％、(87.14±11.42)％、(85.62±9.20)％、(88.41±9.53)％],差异均有统计学意义(P＜0.05).观察组患儿血清IL-33、IL-25水平与Eotaxin水平呈明显正相关(P＜0.05);血清IL-33、IL-25、Eotaxin水平与肺功能呈负相关(P＜0.05).结论 MPP患儿的血清IL-33、IL-25、Eotaxin水平均明显上升,且与肺功能呈负相关.血清IL-33、IL-25、Eotaxin水平可作为MPP患儿肺功能评估的辅助指标.
Abstract
Objective To explore the correlation between serum interleukin(IL)-25,IL-33 and eosinophilic chemotactic factor(Eotaxin)levels and lung function in children with Mycoplasma pneumoniae pneumonia(MPP).Methods Sixty MPP children admitted to Daqing Longnan Hospital from June 2021 to June 2023 were retrospectively selected as the observation group,and 60 healthy children admitted to Daqing Longnan Hospital during the same period were selected as the control group;Blood samples of the two groups were collected,and serologi-cal indexes[CysLTs,ECP,Eotaxin,IL-33,IL-25,IL-6,IL-4,tumor necrosis factor α(TNF-α)]were determined.At the same time,the pulmonary function indexes[tidal volume,time to peak(tPTEF/TE),the ratio of instantaneous flow velocity/tidal expiratory peak flow veloci-ty at 75％tidal volume(TEF25/PTEF％),forced vital capacity(FVC),volume of exhaled air in the first second after maximum deep breathing(FEV1),maximum expiratory peak flow rate(PEF)]were evaluated.The correlation between Eotaxin,IL-33,IL-25 and lung function was analyzed.Results The levels of serum CysLTs,ECP,Eotaxin,IL-33,IL-25,IL-6,IL-4 and TNF-α in the observation group were(637.85±98.42)ng/L,(28.91±4.43)μg/L,(110.96±18.85)ng/L,(11.76±1.53)ng/L,(46.23±7.31)ng/L,(58.54±9.46)pg/mL,(42.81±7.53)ng/L and(3.02±0.89)mg/L,respectively.They were significantly higher than those in the control group[(493.49±78.54)ng/L,(14.95±2.43)μg/L,(101.76±18.53)ng/L,(10.21±0.98)ng/L,(39.62±6.74)ng/L,(24.63±4.58)pg/mL,(26.52±4.49)ng/L,(0.92±0.28)mg/L],the differences were statistically significant(P＜0.05).The tidal volume,tPTEF/TE,TEF25/PTEF％,FVC,FEV1 and PEF in the observation group were(8.01±1.18)mL/kg,(36.16±6.54)％,(66.03±11.34)％,(66.57±10.02)％,(69.91±9.35)％and(73.14±9.83)％,respectively.They were significantly lower than those in the control group[(9.19±1.28)mL/kg,(46.03±6.92)％,(78.63±14.75)％,(87.14±11.42)％,(85.62±9.20)％,(88.41±9.53)％],the differences were statistically significant(P＜0.05).Serum IL-33 and IL-25 were significantly positively correlated with Eotaxin levels(P＜0.05),and serum IL-33,IL-25,and Eotaxin were negatively correlated with lung function(P＜0.05).Conclusion Serum levels of IL-33,IL-25,and Eotaxin all increase significantly in children with MPP and are negatively correlated with lung function.Serum levels of IL-33,IL-25,and Eotaxin can be used as adjuncts for pulmonary function assessment in children with MPP.
基金项目
黑龙江省卫生健康委科研项目(2022060631027)
NETL
NSTL
万方数据