Ki-67和CK7与膀胱癌肿瘤特征的关系及其在复发风险预测中的应用价值
Application value of Ki-67 and CK7 in predicting the stage and recurrence risk of bladder cancer
陈刚 1苏斌杰 1袁兴娥2
作者信息
- 1. 安康市人民医院泌尿外科 陕西 安康 725000
- 2. 安康市人民医院病理科 陕西 安康 725000
- 折叠
摘要
目的 探究Ki-67和细胞角蛋白7(CK7)与膀胱癌肿瘤分期的关系及其在复发风险预测中的应用价值.方法 前瞻性选取2019年9月至2023年11月安康市人民医院收治的98例膀胱癌患者作为研究对象.取膀胱癌组织样本,使用Ki-67和CK7抗体进行免疫组织化学染色,采用半定量方法评价染色效果.分析膀胱癌患者Ki-67和CK7的表达情况与临床病理特征的关系.对65例浅表性膀胱癌患者进行为期2年的随访,分析Ki-67与CK7表达情况与肿瘤复发的关系.采用Pearson相关分析法分析Ki-67与CK7的相关性.结果 Ki-67的表达在性别和年龄中差异均无统计学意义(P＞0.05);随着病理分级的升高,Ki-67的表达呈增加趋势,但差异无统计学意义(P＞0.05);浸润性膀胱癌组织中Ki-67阳性率为81.82％,明显高于浅表性膀胱癌(32.31％),差异有统计学意义(P＜0.05).CK7的表达在性别和年龄中差异均无统计学意义(P＞0.05);随着病理分级的升高,CK7的表达呈降低趋势,但差异无统计学意义(P＞0.05);浸润性膀胱癌组织中CK7阳性率为33.33％,明显低于浅表性膀胱癌(56.92％),差异有统计学意义(P＜0.05).随访结果发现,Ki-67的阳性表达与肿瘤复发显著相关(P＜0.05),而CK7的表达与复发无显著关联(P＞0.05).此外,Ki-67与CK7之间呈显著负相关(r=-0.461,P=0.003).结论 Ki-67和CK7在膀胱癌患者中的表达情况与肿瘤分期和复发风险有显著关联.Ki-67表达水平的提高与膀胱癌的浸润性增强和复发风险增高有关,CK7表达水平的降低则与膀胱癌的浸润性增强相关.Ki-67和CK7可以作为评估膀胱癌分期和复发风险的重要生物标志物.
Abstract
Objective To investigate the relationship between Ki-67 and cytokeratin 7(CK7)and the tumor stage of bladder cancer and its application value in the prediction of recurrence risk.Methods A total of 98 patients with bladder cancer admitted to Ankang People's Hospital from September 2019 to November 2023 were prospectively selected as research objects.The samples of bladder cancer were collected and immunohistochemical staining was performed with Ki-67 and CK7 antibodies.The staining effect was evaluated by semi-quantitative method.The relationship between the expression of Ki-67 and CK7 and clinicopathological features in patients with bladder cancer was analyzed.Sixty-five patients with superficial bladder cancer were followed up for 2 years.The relationship between Ki-67 and CK7 expression and tumor recur-rence was analyzed.The correlation between Ki-67 and CK7 was analyzed by Pearson correlation analysis.Results There was no statistically significant difference in the expression of Ki-67 in gender and age(P＞0.05);with the increase of pathological grade,the expression of Ki-67 showed an increasing trend,but the difference was not statistically significant(P＞0.05);the positive rate of Ki-67 in invasive bladder cancer tissues was 81.82％,which was significantly higher than that in superficial bladder cancer(32.31％),and the difference was statistically significant(P＜0.05).There was no statistically significant difference in the expression of CK7 between gender and age(P＞0.05);with the increase of pathological grade,the expression of CK7 showed a decreasing trend,but the difference was not statistically significant(P＞0.05);the positive rate of CK7 in invasive bladder cancer tissues was 33.33％,which was significantly lower than that in superficial bladder cancer(56.92％),and the difference was statistically significant(P＜0.05).The follow-up results showed that the positive expression of Ki-67 was significantly correlated with tumor recurrence(P＜0.05);there was no significant correlation between CK7 expression and recurrence(P＞0.05).In addition,there was a significant negative correlation between Ki-67 and CK7(r=-0.461,P=0.003).Conclusion The expres-sion of Ki-67 and CK7 in patients with bladder cancer is significantly correlated with tumor stage and recurrence risk.The increase of Ki-67 ex-pression level is associated with the increase of invasive bladder cancer and the increase of recurrence risk,and the decrease of CK7 expression lev-el is associated with the increase of invasive bladder cancer.Ki-67 and CK7 can serve as important biomarkers for assessing the stage and risk of recurrence of bladder cancer.
关键词
膀胱癌/Ki-67/CK7/阳性表达/肿瘤分期/复发Key words
Bladder cancer/Ki-67/CK7/Positive expression/Pathological grade/Recrudescence引用本文复制引用
出版年
2024
NETL
NSTL
万方数据