Purification, Degradation and Scavenging Activities on DPPH Free Radical of Proanthocyanidins from Black Wattle Bark
Abstratct:Proanthocyanidins were extracted from black wattle bark by the ultrasonic technology and purified on AB-8 resin.The oligomers were extracted with ethyl acetate and polymers in water were degraded by H2SO3.Finally,the scavenging activities on 2,2-diphenyl-l-pierylhydrasyl free radical (DPPH·) of each fraction were determined.The results showed that the mass fraction of proanthocyanidins in crude extraction increased from 36.2 ％ to 50.4 ％ by purification on AB-8 resin.The recovery was 90.3 ％.The proanthocyanidins were degraded by H2SO3 significantly and the average degree of polymerization drop to 6.25 from 8.11 after degradation.It was found that the proanthocyanidins after degradation had higher DPPH scavenging activity and EC5o reduced from 2.25 ％ to 1.88 ％.
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NSTL
万方数据
维普
