Preparation of Hydrolysates from Ginkgo biloba Seed Protein Isolate and Their Antioxidant Activity
Ginkgo biloba seed protein isolate(GBSPI)was hydrolyzed by alcalase,bromelain,and Aspergillus oryzae protease,and the antioxidant activity of the hydrolysates was evaluated through in vitro DPPH radical,hydroxyl radical,and superoxide anion radical scavenging experiments.The results showed that the yields of hydrolysates by alcalase,bromelain,and Aspergillus oryzae protease were 93.60%,60.00%and 46.80%,with degrees of hydrolysis were 13.76%,6.28%and 5.55%,respectively.The hydrolysates from three enzymes exhibited good inhibitory effects on DPPH radical,hydroxyl radical,and superoxide anion radical.Among them,the hydrolysate from alcalase showed the strongest antioxidant activity,with IC50 values of 5.29 g/L for DPPH radical,0.35 g/L for hydroxyl radical,and 7.01 g/L for superoxide anion radical,respectively.
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