Commercially available G.sinensis saponin(GSS)had deep color,heavy odor,and low saponin content,which limited its application.In this paper,the solvent extraction of GSS was separated and purified by H2O2 decolorization and AB-8 macroporous adsorption resin column chromatography.The purity,structure composition,surface activity,foam and emulsification properties of saponin were compared and studied.The results showed that the structure of saponin purified by H2O2 decolorization(H2O2-GSS)was damaged,the saponin content was 48.02%,but the foam stability was excellent and the half-life(t1/2)was 10 h.Results of column chromatography showed that the highest purity of saponin was achieved through elution using 60%ethanol,resulting in a 92.12%saponin mass fraction.Additionally,the foam performance was the best,reaching a height of up to 11 cm,and the emulsion was the most stable within 24 h.After 24 hours,stratification occurred at a height of 2.2 cm.The mass fractionofsaponinelutedby75%ethanolwas86.40%,thesurfacetensionvaluewasthelowest(41.50 mN/m),and the critical micelle concentration(CCMC)was 3 g/L(having the best surface activity).The H2 O2-GSS showed better foam stabilization ability.The GSS obtained by column chromatography had higher purity,better surface activity,foam performance and emulsion stability.The surface activity of purified recombinant saponin(M-GSS)was further improved,which provided ideas for the development of high-performance surfactants.
关键词
皂苷/分离纯化/AB-8/大孔吸附树脂/表面活性/稳泡性能
Key words
saponins/separation and purification/AB-8 macroporous adsorption resin/surface activity/bubble stability
万方数据