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生物炭作为外源乳酸菌载体对构树青贮饲料的影响

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在构树青贮饲料发酵过程中,评估了生物炭作为外源乳酸菌载体对构树青贮饲料发酵品质与营养成分的影响。构树青贮饲料的营养成分分析结果显示:生物炭与蔗糖的协同作用显著提高了构树青贮饲料中干物质(DM)含量,75 d时生物炭/蔗糖/菌剂(BS)组的DM质量分数达到47。60%(基于鲜质量),较菌剂(CN)组提高了 4。32%,从而改善了青贮饲料的保存效果和营养保留率。蔗糖/菌剂(CS)组在发酵初期含粗蛋白10。54%,较CN组降低了 0。62%,表明蔗糖的添加可能加速了蛋白质降解。此外,生物炭与蔗糖联合使用显著降低了构树青贮饲料中纤维素(CE)含量,75 d时CE质量分数降至24。71%,较CN组降低了 3。11%,提高了发酵质量。化学组分分析结果表明:生物炭与蔗糖联合使用,在发酵初期显著提高了构树青贮饲料中乳酸含量,3 d时乳酸量升高至6。73%,比CN组高出32。16%,表明该处理方法能有效促进乳酸菌的生长和代谢活动。生物炭/菌剂(BN)组在整个发酵期,其NH3-N含量显著高于CN组的,且在第75天达到峰值4。3%,但仍远低于优质青贮饲料的最大限值(10%,以总氮计),表现出优质青贮饲料的特性。生物炭作为外源乳酸菌载体显著改善了构树青贮饲料的发酵品质,且对营养成分无负面影响,其中生物炭与蔗糖的联合使用效果最佳。
The Impact of Biochar as a Carrier for Exogenous Lactic Acid Bacteria on Paper Mulberry Silage
During the fermentation process of paper mulberry(Broussonetia papyrifera(L.))silage,the study evaluated the effect of biochar as a carrier for exogenous lactic acid bacteria on the fermentation quality and nutrient composition of silage made from paper mulberry.The results of nutrient composition analysis of the paper mulberry silage showed that the synergistic effect of biochar and sucrose(BS)increased the dry matter content of paper mulberry silage to 47.60%(based on fresh quality)at 75 d,which was 4.32%higher than that of bacteria-only(CN)group and improved its silage preservation and nutrient retention rate.At the initial stage of fermentation,the sucrose treatment(CS)of paper mulberry silage had a crude protein content of 10.54%,which was 0.62%lower than that CN group,suggesting that the addition of sucrose might accelerate the degradation of protein.Additionally,the combined use of biochar and sucrose reduced the paper mulberry silage cellulose content to 24.71%at 75 d,which was 3.11%lower than bacteria-only group,enhancing its fermentation quality.Chemical composition analysis results showed that the combining of biochar and sucrose significantly increased the lactic acid content in paper mulberry silage at the early stage of fermentation.The lactic acid content increased to 6.73%DM at 3 d,which was 32.16%higher than CN group,indicating that this treatment method could effectively promote the growth and metabolic activities of lactic acid bacteria.The NH3-N content of the biochar and bacteria agent(BN)group was higher than CN group throughout fermentation,peaking at 4.3%on the 75th day,but it was still well below the high-quality silage maximum limit(10%in total nitrogen),indicating high-quality silage characteristics.In conclusion,biochar as a carrier for exogenous lactic acid bacteria notably improved the fermentation quality of paper mulberry silage without negatively affecting nutrient composition,with the best effects from combining biochar and sucrose.

anaerobic fermentationbiocharfermentation pathwayssustainable agriculture

曹景文、王学凯、杨富裕

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中国农业大学草业科学与技术学院,北京 100193

中国农业大学动物科学技术学院,北京 100193

贵州大学动物科学学院,贵州贵阳 550025

厌氧发酵 生物炭 发酵途径 可持续农业

国家自然科学基金资助项目中国博士后科学基金

322014732022M723420

2024

林产化学与工业
中国林业科学研究院林产化学工业研究所 中国林学会林产化学化工分会

林产化学与工业

CSTPCD北大核心
影响因子:0.696
ISSN:0253-2417
年,卷(期):2024.44(5)