The Impact of Biochar as a Carrier for Exogenous Lactic Acid Bacteria on Paper Mulberry Silage
During the fermentation process of paper mulberry(Broussonetia papyrifera(L.))silage,the study evaluated the effect of biochar as a carrier for exogenous lactic acid bacteria on the fermentation quality and nutrient composition of silage made from paper mulberry.The results of nutrient composition analysis of the paper mulberry silage showed that the synergistic effect of biochar and sucrose(BS)increased the dry matter content of paper mulberry silage to 47.60%(based on fresh quality)at 75 d,which was 4.32%higher than that of bacteria-only(CN)group and improved its silage preservation and nutrient retention rate.At the initial stage of fermentation,the sucrose treatment(CS)of paper mulberry silage had a crude protein content of 10.54%,which was 0.62%lower than that CN group,suggesting that the addition of sucrose might accelerate the degradation of protein.Additionally,the combined use of biochar and sucrose reduced the paper mulberry silage cellulose content to 24.71%at 75 d,which was 3.11%lower than bacteria-only group,enhancing its fermentation quality.Chemical composition analysis results showed that the combining of biochar and sucrose significantly increased the lactic acid content in paper mulberry silage at the early stage of fermentation.The lactic acid content increased to 6.73%DM at 3 d,which was 32.16%higher than CN group,indicating that this treatment method could effectively promote the growth and metabolic activities of lactic acid bacteria.The NH3-N content of the biochar and bacteria agent(BN)group was higher than CN group throughout fermentation,peaking at 4.3%on the 75th day,but it was still well below the high-quality silage maximum limit(10%in total nitrogen),indicating high-quality silage characteristics.In conclusion,biochar as a carrier for exogenous lactic acid bacteria notably improved the fermentation quality of paper mulberry silage without negatively affecting nutrient composition,with the best effects from combining biochar and sucrose.