Effect of Gallates With Different Length of Carbon Chain on Skin Wound Healing in Mice
In order to investigate the effect of gallates with different lengths of carbon chain on skin wound healing,the mechanical injury model of the whole skin on the back of mouse was adopted,and the representative short-chain propyl gallate(GA-C3),long-chain gallate octadecyl ester(GA-C18)and ultra-long carbon chain gallate octacosyl ester(GA-C28)were selected as experimental drugs to treat the wound,and both model and blank groups were set up.The effect of gallates on the wound healing was investigated by measuring the rate of wound healing,observing tissue sections,detecting interleukin-1β(IL-1β),interleukin-6(IL-6),hepatocyte growth factor(HGF),fibroblast growth factor-β(FGF-β),transforming growth factor-β1(TGF-β1),vascular endothelial growth factor(VEGF),signal transducer and activator of transcription 3(STAT3),Src protein(Src)and so on.The results showed that the use of different gallates at the wound site could promote wound healing on mouse in the first 7 days.GA-C18 with a long carbon chain had a slightly better effect than short carbon chain GA-C3 and super long carbon chain GA-C28,but there was no significant difference between the above three.On day 14,the wound healing rate of the mice in three drugs groups was more than 98.5％,which was significantly better than that of the model group(94.3％).Tissue sections showed that the gallate-treated wound tissue had a low inflammatory response on day 3 and more abundant granulation tissue,fibrocytes and neo vasculature on day 7 than the model group.In addition,the cytokine detection results showed that the gallates used in the wound could stimulate the animal body to secrete more factors such as IL-1β,IL-6,HGF,FGF-β,TGF-β1,VEGF,STAT3 and Src within 3 and 7 days,thereby accelerating the transition from the inflammatory stage of wound healing to the proliferative and remodelling stage,reducing the inflammatory response and promoting wound healing.
gallatewound healinggrowth factorcarbon chain lengthinjury model
万方数据
维普
