Analysis of potentially co-expressed hub genes and molecular mechanism for alcohol use disorders and schizophrenia based on bioinformatics method core gene screening and molecular mechanism analysis
Objective:To investigate the common gene expression characteristic and molecular mechanism of alcohol use disorder(AUD)and schizophrenia(SCZ)using bioinformatics methods.Method:The AUD gene expression dataset GSE161986 as well as SCZ gene expression datasets GSE53987,GSE17162,and GSE21138 were downloaded from Gene Expression Omnibus(GEO)database.Data normalization and Differentially Ex-pressed Genes(DEGs)screening were carried out both in AUD and SCZ datasets.Based on these DEGs,function-al annotation and pathway analysis were performed using the Database for Annotation,Visualization and Integrated Discovery(DAVID).Protein-Protein Interaction(PPI)network construction and key genes screening were ana-lyzed using the Search Tool for the Retrieval of Interacting Genes(STRING)database and Cytoscape software.GSE44456 and GSE87610 were applied for potential core genes validation.Results:A total of 95 DEGs were i-dentified.Functional annotation and pathway analysis indicated that up-regulated DEGs were mainly involved in negative regulation of apoptosis and NF-kappa B signal pathway.Down-regulated DEGs were mainly related to chemical synaptic transmission and neuroactive ligand-receptor interaction pathways.Metallothionein gene(MTIG),MT2A,and parvalbumin(PVALB)may played a key role in the co-occurrence of AUD and SCZ and the pathogenesis of alcohol-induced mental disorders.Conclusion:Aberrant expression of MTIG,MT2A,and PVALB may play a key role in the co-occurrence of AUD and SCZ,as well as the pathogenesis of alcohol-induced mental disorders.These genes may serve as potential molecular targets for the prevention and treatment of these related diseases.
alcohol use disorderschizophreniadifferentially expressed geneshub genes
NETL
NSTL
万方数据
