Disinfection effects of 2％chlorhexidine alcohol spray on mice abdominal surgical incision:a randomized controlled trial
Objective:To clarify the disinfection effects of 2％chlorhexidine alcohol spray on mice abdominal surgical incisions,in order to provide a new disinfection choice for abdominal patients with acute abdomen surgery in clinical.Methods:Eighty healthy C57BL/6 male mice(6-8 weeks old)were randomly divided into four groups:control group,experimental group A,experimental group B,and experimental group C,with 20 mice in each group.Mice in the control group were rubbed with 2％chlorhexidine solution on the abdomen for 3 times to disinfect,while mice in the experimental group A were sprayed with 2％chlorhexidine solution twice,mice in the experimental group B were sprayed for 3 times,and mice in the experimental group C were sprayed for 4 times.The median abdominal incision was applied to simulate the human abdominal surgical process.The time of disin-fection,the volume of disinfectant,the amount of colonies cultured on the edge of the incision for pre-operation and post-operation,the amount of colonies cultured on the abdominal lavage fluid smear for the 7th day,and the postoperative complications within 7 days were compared among the four groups.Results:Compared with the control group,experimental groups A,B and C showed shorter disinfection time and less volume of disinfectant(P＜0.01).As for the colony counts of bacteria cultured from the intended incision sites for one minute before disinfection,there was no significant difference within the four groups(P＞0.05).The amounts of colonies in ex-perimental group A was more than that in the control group at 5 minutes after disinfection,30 and 60 minutes during operation,2 and 48 hours after operation(P＜0.01),but there was no significant difference among experi-mental group B,C and control group(P＞0.05).The total amount of post-operative complications within 7 days in experimental group A was more than that in the control group(P＜0.05),but there was no significant differ-ence among experimental group B,C and the control group(P＞0.05).Conclusion:For the disinfection of 2％chlorhexidine alcohol solution in the abdominal surgical site of mice,three or four times of spraying can achieve the same disinfection effects as three times of rubbing.Furthermore,the spraying method shows shorter disinfec-tion time and less volume of disinfectant.Therefore,2％chlorhexidine alcohol spray disinfection has a great pros-pect in the disinfection of abdominal surgery especially for emergency surgery patients.
NETL
NSTL
万方数据
