Significance of changes of lymphocyte subsets and their ratios in patients with sepsis in immune regulation
Objective:To explore the significance of the changes of lymphocyte subsets and their ratios in pa-tients with sepsis in immune regulation.Methods:This was a single-center,cross-sectional study.The subjects of the study were 55 sepsis patients admitted to our hospital from January 2020 to March 2022 and 50 age-matched healthy controls recruited.According to the definition of sepsis and septic shock,patients with sepsis were further divided into sepsis group(39 cases)and septic shock group(16 cases).CD4+T lymphocytes,CD8+T lympho-cytes,B lymphocytes and NK cells in peripheral blood were quantified by flow cytometry.Results:The percenta-ges of CD8+T lymphocytes and NK cells in the sepsis group were significantly lower than those in control group(P＜0.05).The percentage of NK cells in septic shock group was significantly lower than that in control group(P＜0.05).The percentage of B lymphocytes,CD4+T/CD8+T,and B/NK in the sepsis group were significantly higher than those in control group and septic shock group(P＜0.05).The percentage of CD8+T lymphocytes,T/B,CD4+T/B,and CD8+T/B in sepsis group were significantly lower than those in the control group and septic shock group(P＜0.05).The AUC of B lymphocyte percentage,CD4+T/CD8+T,T/NK,CD4+T/NK,CD8+T/NK,and B/NK in the diagnosis of sepsis were 0.609,0.560,0.671,0.645,0.602,and 0.680,respectively,with the critical values of 18.6,1.57,4.89,3.05,2.18,and 1.16,respectively.The AUC of T lymphocyte per-centage,CD8+T lymphocyte percentage,T/B,CD4+T/B,CD8+T/B,and CD8+T/NK in the diagnosis of septic shock were 0.649,0.739,0.680,0.600,0.725,and 0.628,respectively,with the cutoff values of 73.7,26.8,6.25,3.57,2.26,and 4.28,respectively.Conclusion:The pattern of immune expression in patients with sepsis is not a simple decrease in the number of lymphocytes,as changes in the functional ratio of lymphocyte subsets may be more critical in the progression of sepsis.
万方数据
维普
