A study of the diagnostic value of peripheral blood polygenic association in patients with sepsis
Objective:To investigate the diagnostic value of peripheral blood polygenic association in patients with sepsis.Methods:Fifty-one patients with sepsis and 30 patients with non-infectious systemic inflammatory re-sponse syndrome(SIRS)admitted to the Department of Intensive Care Medicine(ICU)of Baotou Central Hospi-tal from May 2023 to April 2024 were selected,and 30 physically examined healthy people were also selected as the control group.Clinical data of all patients on the first day of admission were collected,and the expression lev-els of CCAAT/enhancer binding protein epsilon(CEBPE),ubiquitin-conjugating enzyme E2-S(UBE2S),cell cy-cle-dependent protein kinase 5(CDK5),lipid transfer protein 10(STARD10),and Rab11-GTP-binding protein(RELCH)in peripheral blood were detected.Spearman's correlation analysis was used to analyze the correlation between multiple genes and inflammatory indicators and clinical scores.In addition,the risk factors for sepsis were analyzed by multifactorial binary logistic regression,and the diagnostic value of single genes and multiple genes in combination for sepsis was evaluated by plotting the working characteristic(ROC)curve of the subjects.Results:The white blood cell count(WBC),procalcitonin(PCT),C-reactive protein(CRP),lactate(Lac),PH,acute physiology and chronic health status(APACHEⅡ)score and sequential organ failure(SOFA)score in the sepsis group were statistically significant different from those in non-infected SIRS group(P＜0.05).The expres-sion levels of CEBPE and UBE2S genes were significantly higher in the sepsis group compared with the normal control group and the non-infected SIRS group(P＜0.001).The expression levels of CDK5 gene were significantly higher in the sepsis group than in the non-infected SIRS group(P＜0.001),but there was no statistically signifi-cant difference when compared with the normal control group(P＞0.05).There were no significant differences in the expression levels of STARD10 and RELCH genes among the three groups(P＞0.05).Spearman correlation a-nalysis showed that PCT,CRP and clinical scores were significantly positively correlated with the expression levels of CEBPE and UBE2S genes(r＞0,P＜0.05),but were not significantly correlated with the expression level of CDK5 gene(P＞0.05).Binary logistic regression analysis showed that SOFA score and UBE2S gene were inde-pendent risk factors for the development of sepsis in ICU patients(P＜0.05).ROC curve analysis showed that the AUC of CEBPE,UBE2S and CDK5 genes for diagnosing sepsis were 0.861,0.909 and 0.725,respectively,and the AUC of the combined diagnosis of the three genes was 0.939,and the sensitivity and specificity were 0.902 and 0.867,respectively.The AUC of the three genes in combination with the clinical SOFA score for the diagno-sing of sepsis was(0.973)was the largest,and the sensitivity and specificity of the diagnosis were 0.902 and 0.967.Conclusion:The combined diagnostic efficacy of CEBPE,UBE2S,and CDK5 polygenes in peripheral blood of patients with sepsis is significantly higher than that of individual genes,and the combination of multiple genes and clinical SOFA score has higher diagnostic value.
万方数据
维普
