新生及成年大鼠七氟醚 MAC 值的测定
Determination of sevoflurane minimum alveolar concentration in newborn and adult rats
黄艳 1杨小霖 2黄三2
作者信息
- 1. 637000 四川省南充市中心医院麻醉科
- 2. 川北医学院附属医院麻醉科
- 折叠
摘要
目的：测定并比较新生及成年大鼠七氟醚的最低肺泡有效浓度(MAC)。方法选择健康新生及成年 SD 大鼠各25只。两组大鼠分别放入自制大鼠麻醉装置，露出其尾部。七氟醚气体经麻醉机挥发罐输入麻醉装置。采用序贯法及夹尾刺激测定七氟醚 MAC。七氟醚初始测定浓度为1.5%，调节梯度为±0.2%。每间隔20 min 重复夹尾试验判断麻醉效果并抽取气体样本3~5 ml 测定七氟醚浓度。如此反复，使每只大鼠均获得一个阳性与阴性反应的交叉点。组内每只大鼠 MAC值的均值即为该组大鼠的 MAC 值。结果新生大鼠 MAC 值为(2.58±0.11)%，明显高于成年大鼠的 MAC 值(2.32±0.13)%(P <0.01)。结论新生大鼠的七氟醚 MAC 值明显高于成年大鼠，达到同一麻醉深度时新生大鼠所需七氟醚浓度更高。
Abstract
Objective To measure and compare sevoflurane minimum alveolar concentration (MAC)in newborn and adult rats.Methods The rats were divided into newborn rat (6-8 days) group (25 cases)and adult rat (8-10 weeks)group (25 cases).All rats were settled in the self-made device for anesthesia with inhaled sevoflurane,and the tails of rats were exposed out of the device. Sevoflurane was given via an anesthesia machine.Up-and-down method and clamping tail stimulus were applied to measure the MAC values of sevoflurane in the two groups.1.5% of sevoflurane was set as the initial concentration,and± 0.2% as adjustable gradient.A positive or negative response was judged by clamping tail stimulus in a 20 minutes interval.The mean MAC value of all rats in each group was defined as the MAC value.Results The MAC value of newborn rats was (2.58 ± 0.1 1)%,and the MAC value of adult rats was (2.32 ±0.13)%.A significant difference was found between the two groups (P <0.01).Conclusion The MAC value of newborn rats was much higher than that of adult rats.Newborn rats need a higher concentration of sevoflurane at the same depth of anesthesia when compared with adult rats.
关键词
七氟醚/新生大鼠/成年大鼠/最低肺泡有效浓度Key words
Sevoflurane/Newborn rat/Adult rat/Minimum alveolar concentration (MAC)引用本文复制引用
出版年
2016
NETL
NSTL
万方数据