多血型系统抗体鉴定和交叉配血策略一例抗-f合并抗-M
Multi-blood Group System Antibody Identification and Cross-matching Strategies:One Case of Anti-f Combined with Anti-M
郑仲男 1李晓菲 1张烨 1苗天红1
作者信息
摘要
目的 鉴定一例患者血浆中抗体的特异性并制定交叉配血的策略.方法 采用盐水介质试管法和抗人球蛋白卡(LISS/Coombs卡)做抗体筛选和抗体鉴定,根据谱细胞、酶处理谱细胞及4℃谱细胞反应格局,确定患者血浆中意外抗体的特异性.结果 患者Rh血型为CCDee,MN血型为NN.多种抗体鉴定结果显示,其血浆中存在同种抗体:Rh血型系统抗-f(f:ce复合抗原)合并MNS血型系统抗-M.结论 抗-f仅与具有f抗原(R0、r)的红细胞反应,且能被蛋白水解酶增强;IgG类抗-M在4℃环境的反应性更强.经过输血免疫后,患者产生抗-f和抗-M抗体,交叉配血策略为优先选择无M抗原红细胞进行交叉配血,对配血相合的红细胞成分血进行RhCE抗原检测,选择无f抗原红细胞发血.
Abstract
Objective To identify the specificity of antibodies in plasma of patients and develop strategies for cross matching.Methods Antibodies were screened and identified by saline medium test tube method and LISS/Coombs card,and the specificity of plasma antibodies was determined by the reaction patterns of the Makropanel 16,the Makropanel 16-P and the panel cell at 4℃.Results The patient's Rh blood group was CCDee and MN blood group was NN.Multiple antibody identification results showed that there were alloantibodies in the plasma:anti-f(f:ce composite antigen)of Rh blood group system and anti-M of MNS blood group system.Conclusion Anti-f only reacted with red blood cells containing f antigen(R0,r)and can be enhanced by proteolytic enzymes.The IgG anti-M showed stronger reactivity in the 4℃environment.After the transfusion and immunization,the patient produced anti-f and anti-M antibodies.In clinical blood matching,red blood cells without M antigen were preferentially selected for cross matching,RhCE antigen was detected according to blood matching results,and red blood cells without f antigen were selected for transfusion.
关键词
抗体鉴定/RhCE复合抗原/f抗原/抗-f/抗-MKey words
Antibody identification/RhCE composite antigen/f antigen/Anti-f/Anti-M引用本文复制引用
出版年
2024
国家科技期刊平台
NSTL
万方数据