Exploration of the Mechanism of Sema7a Promoting Adhesion of Umbilical Vein Endothelial Cells to Megakaryocytes
Objective The purpose of this study was to explore the mechanism of Sema7a protein promoting the adhesion between endothelial cells and megakaryocytes.Methods Human umbilical vascular endothelial cells(HUVECs)and megakaryocyte line MEG01 were used to simulate the adhesion of pulmonary vessels and megakaryocytes in vitro.Immunofluorescence,flow cytometry,4D Label free and other biological techniques were used to detect the adhesion between endothelial cells and megakaryocytes,the amount of Sema7a binding to HUVECs,and the changes of protein expression and biological information in HUVECs.Immunofluorescence was used to detect the expression of intercellular cell adhesion molecule-1(ICAM-1)and vascular cell adhesion molecule-1(VCAM-1)in HUVECs.Results The adhesion between MEG01 and HUVECs was promoted after Sema7a binding to HUVECs,with the MAPK signal pathway been activated,and molecules ICAM-1 and VCMA-1 of HUVECs been up-regulated.Conclusion Sema7a promoted the adhesion between megakaryocytes MEG01 and endothelial cells HUVECs by up-regulating the expression of molecules ICAM-1 and VCMA-1 in HUVECs.
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